CAJ | 학술논문

中国线辣椒(C.annuum var.annuum)是一种特有的辣椒类型,具有丰富的种质资源,但目前对其遗传多样性的研究甚少.本研究采用 RAPD和 SSR分子标记,对20份辣椒种质资源(其中线辣椒13份,其他辣椒资源7份)的遗传多样性进行鉴定分析,并比较分析了形态特征.结果表明：2种标记均能有效鉴定材料间遗传多样性,其中 SSR标记优于 RAPD标记.RAPD标记共扩增出218条 DNA 片段,每个引物平均可扩增出3~10条 DNA 片段,多态性比率(PPB)为77.1%；材料间遗传相似系数(GS)为0.615~0.905.SSR 标记共扩增出153条 DNA 片段,每对引物平均扩增出2~8条DNA片段,多态性比率(PPB)为82.3%；材料间遗传相似系数(GS)为0.632~0.886.相关性分析表明,RAPD和 SSR标记与形态标记的相关系数分别为0.743和0.710,2种标记与形态标记的相关系数为0.658,均达到了显著水平.聚类分析结果显示,大部分具有亲缘关系的资源及形态学、生物学特征相近的资源聚在一类,说明分子标记的聚类分析结果与形态学分类结果具有较好的相符性.
중국선랄초(C.annuum var.annuum)시일충특유적랄초류형,구유봉부적충질자원,단목전대기유전다양성적연구심소.본연구채용 RAPD화 SSR분자표기,대20빈랄초충질자원(기중선랄초13빈,기타랄초자원7빈)적유전다양성진행감정분석,병비교분석료형태특정.결과표명：2충표기균능유효감정재료간유전다양성,기중 SSR표기우우 RAPD표기.RAPD표기공확증출218조 DNA 편단,매개인물평균가확증출3~10조 DNA 편단,다태성비솔(PPB)위77.1%；재료간유전상사계수(GS)위0.615~0.905.SSR 표기공확증출153조 DNA 편단,매대인물평균확증출2~8조DNA편단,다태성비솔(PPB)위82.3%；재료간유전상사계수(GS)위0.632~0.886.상관성분석표명,RAPD화 SSR표기여형태표기적상관계수분별위0.743화0.710,2충표기여형태표기적상관계수위0.658,균체도료현저수평.취류분석결과현시,대부분구유친연관계적자원급형태학、생물학특정상근적자원취재일류,설명분자표기적취류분석결과여형태학분류결과구유교호적상부성.
The Chinese Xianlajiao (Capsicumannuum var.annuum)is a unique type of pepper and has a rich germplasm.However,there is very little research on its genetic diversity.The genetic diversity of 20 Capsicumgermplasm accessions (Xianlajiao accessions of 13,other accessions 7)was analyzed by means of RAPD and SSR.At the same time,the morphological characteristics were compared and analyzed.The re-sults showed that two markers were effective identification of genetic diversity and the SSR markers was better than that of RAPD marker.2 1 8 DNA fragments were amplified by RAPD markers,each primer am-plified 3 to 10 DNA fragments,polymorphic ratio (PPB)77.1%;the genetic similarity coefficient (GS) ranged from 0.615 to 0.905.153 DNA fragments were amplified by SSR markers,each primer amplified 2 to 8 DNA fragments,polymorphic ratio (PPB)82.3%;the genetic similarity coefficient (GS)ranged from 0.632 to 0.886.Correlation analysis showed that,the correlation coefficient RAPD and SSR markers and morphological markers were 0.743 and 0.710,the correlation coefficient of two markers and morphological markers for 0.658,reached a significant level.Cluster analysis results showed that,most of the germplasm with genetic relationship,similar biological characteristics and morphological characteristics closed together in one class,that the result of cluster analysis of molecular markers and morphological classification results agree.