CAJ | 학술논문

目的：探讨EB病毒( Epstein-Barr virus, EBV)与乳腺癌发生、发展的相关性。方法随机选取不同发展阶段的乳腺病变组织246例,采用聚合酶链反应( polymerase chain reaction, PCR)、原位杂交( in situ hybridization, ISH)、激光捕获显微切割( laser capture microdissection, LCM)、免疫组化染色EnVision法检测EBV DNA、RNA、蛋白质水平,分析EBV与乳腺癌发生、发展的关系。结果免疫组化标记246例乳腺病变均未检测到EBV潜伏膜蛋白LMP1的表达。 PCR法在乳腺癌(12/23)、原位癌(0/10)以及乳腺良性病变(0/15)中检测到EBV DNA,但用地高辛标记的EBV DNA探针对48例乳腺良、恶性病变组织(包括PCR扩增阳性的12例乳腺癌标本)进行ISH检测,在癌细胞、乳腺上皮细胞和间质淋巴细胞内均未检测到阳性杂交信号。采用LCM PCR检测12例PCR扩增阳性和12例阴性乳腺标本的乳腺上皮细胞和间质淋巴细胞,在12例PCR阳性标本的间质细胞中扩增出EBV DNA,癌组织中未检出EBV DNA。用EBV RNA探针和ISH方法在75例乳腺良恶性病变组织中均未检测到EBER表达。结论在该文选择的标本中,乳腺癌发生、发展与EBV感染无关。
목적：탐토EB병독( Epstein-Barr virus, EBV)여유선암발생、발전적상관성。방법수궤선취불동발전계단적유선병변조직246례,채용취합매련반응( polymerase chain reaction, PCR)、원위잡교( in situ hybridization, ISH)、격광포획현미절할( laser capture microdissection, LCM)、면역조화염색EnVision법검측EBV DNA、RNA、단백질수평,분석EBV여유선암발생、발전적관계。결과면역조화표기246례유선병변균미검측도EBV잠복막단백LMP1적표체。 PCR법재유선암(12/23)、원위암(0/10)이급유선량성병변(0/15)중검측도EBV DNA,단용지고신표기적EBV DNA탐침대48례유선량、악성병변조직(포괄PCR확증양성적12례유선암표본)진행ISH검측,재암세포、유선상피세포화간질림파세포내균미검측도양성잡교신호。채용LCM PCR검측12례PCR확증양성화12례음성유선표본적유선상피세포화간질림파세포,재12례PCR양성표본적간질세포중확증출EBV DNA,암조직중미검출EBV DNA。용EBV RNA탐침화ISH방법재75례유선량악성병변조직중균미검측도EBER표체。결론재해문선택적표본중,유선암발생、발전여EBV감염무관。
Purpose To study the relationship between Epstein-Barr virus ( EBV) and breast cancer. Methods 246 cases of breast lesions at different development stages were selected and EBV DNA, RNA and protein was used by polymerase chain reaction ( PCR) , in situ hybridization ( ISH) , laser capture microdissection ( LCM) , immunohistochemistry ( IHC) EnVision technology. Results No expression of EBV latent membrane protein LMP1 was detected in all 246 cases of benign and malignant breast lesions. In 12 cases of breast cancer of EBV DNA, carcinoma in situ and breast lesions not EBV DNA was detected by PCR. However, using digoxigenin la-beled EBV DNA probe for the 48 cases ( including 12 cases of breast cancer specimens of positive PCR amplification) of benign and malignant breast lesions, no positive hybridization signal was detected in cancer cells, mammary epithelial cells and stromal lympho-cytes. Using laser capture microdissection and PCR amplification, cancer cells and stromal cells were captured respectively from 12 ca-ses of PCR positive and 12 cases of PCR negative of breast cancer specimens, we found EBV DNA was only amplified in mesenchymal cells. In the detection of the EBER expression with EBV RNA probe and in situ hybridization, the results of 75 cases of benign and malignant breast lesions ( including 12 cases of breast cancer by positive PCR amplification) were all negative. Conclusions The re-sults indicate that the tumorigenesis and development of breast cancer have nothing to do with EBV infection in all cases were chosen.