CAJ | 학술논문

目的：检测两株人乳腺癌细胞BT474和BT474/siRNA HER-2微小RNA( miRNAs)的表达,初步筛选与HER-2相关差异性表达的miRNAs。方法用干扰RNA技术沉默人乳腺癌细胞株BT474中HER-2基因的表达,利用miRNA芯片技术比较两株细胞BT474和BT474/siRNA HER-2内miRNAs的表达。结果差异倍数>2且P<0．05,与HER-2相关的miRNAs有338个；其中110个miRNAs上调,228个miRNAs下调。结论用miRNA芯片技术可以筛选人乳腺癌BT474细胞在不同HER-2水平差异性表达的miRNAs,进一步分析筛选,有望在乳腺癌中寻找到与HER-2相关的miRNAs。
목적：검측량주인유선암세포BT474화BT474/siRNA HER-2미소RNA( miRNAs)적표체,초보사선여HER-2상관차이성표체적miRNAs。방법용간우RNA기술침묵인유선암세포주BT474중HER-2기인적표체,이용miRNA심편기술비교량주세포BT474화BT474/siRNA HER-2내miRNAs적표체。결과차이배수>2차P<0．05,여HER-2상관적miRNAs유338개；기중110개miRNAs상조,228개miRNAs하조。결론용miRNA심편기술가이사선인유선암BT474세포재불동HER-2수평차이성표체적miRNAs,진일보분석사선,유망재유선암중심조도여HER-2상관적miRNAs。
Purpose To detect the expression profile of microRNAs ( miRNAs) in human breast cancer cells BT474 and BT474/ siR-NA HER-2, and to screen out miRNA expression profile related to HER-2. Methods RNA interference was used to inhibit HER-2 expression in human breast cancer line BT474. The expression profile of miRNAs was detected by miRNA microarray technique. Re-sults According to discrepancy power >2. 0 and P value<0. 05, there were 338 miRNAs related to HER-2, including 110 up-regu-lated and 228 down-regulated miRNAs. Conclusion miRNA microarrays can detect the differentially expressed miRNAs in human breast cancer BT474 cells with different level of HER-2. The miRNAs related to HER-2 which play a role in breast cancer should be found after further analysis.