CAJ | 학술논문

目的：分析肺癌EGFR基因第18~21号外显子突变,以Sanger测序法为参照,探讨xTAG液相芯片用于临床样本检测的适用性。方法随机选择1139例Ⅰ~Ⅳ期肺癌组织标本,提取DNA,分别采用xTAG液相芯片法和Sanger测序法检测EG-FR基因第18~21号外显子突变情况,评价xTAG液相芯片法检测的敏感性和特异性。结果液相芯片法：共1134例患者获得基因突变检测结果,Sanger测序法：共1105例患者获得基因突变检测结果,检测成功率分别为99．56%和97．01%。以San-ger测序法为参照,xTAG液相芯片法检测的敏感性和特异性分别为99．59%和94．54%,两种方法均检出少数样本存在双外显子突变。两种方法检测出的突变型别完全吻合。结论采用xTAG液相芯片法可有效检测肺癌EGFR基因突变,实现突变分型,且相对测序法更方便、高效,适合临床推广应用。
목적：분석폐암EGFR기인제18~21호외현자돌변,이Sanger측서법위삼조,탐토xTAG액상심편용우림상양본검측적괄용성。방법수궤선택1139례Ⅰ~Ⅳ기폐암조직표본,제취DNA,분별채용xTAG액상심편법화Sanger측서법검측EG-FR기인제18~21호외현자돌변정황,평개xTAG액상심편법검측적민감성화특이성。결과액상심편법：공1134례환자획득기인돌변검측결과,Sanger측서법：공1105례환자획득기인돌변검측결과,검측성공솔분별위99．56%화97．01%。이San-ger측서법위삼조,xTAG액상심편법검측적민감성화특이성분별위99．59%화94．54%,량충방법균검출소수양본존재쌍외현자돌변。량충방법검측출적돌변형별완전문합。결론채용xTAG액상심편법가유효검측폐암EGFR기인돌변,실현돌변분형,차상대측서법경방편、고효,괄합림상추엄응용。
Purpose To analyze EGFR exon 18 ~21 gene mutations in lung cancer, and compare xTAG liquidchip technology and Sanger sequencing technology in clinical practice. Methods 1 139 tumor tissue samples from phaseⅠtoⅣlung cancer patients were randomly collected. DNA was extracted from the samples. EGFR gene mutation status in exon 18~21 was detected by xTAG liquidchip technology and Sanger sequencing technology respectively. Results The mutation status of EGFR was obtained by xTAG liquidchip technology in 1 134 patients, and 1 105 by Sanger sequencing technology, detection success rate was 99. 56% and 97. 01% respective-ly. The sensitivity and specificity of xTAG liquidchip technology Comparing with Sanger sequencing was 99. 59% and 94. 54%. Sever-al cases of multiple mutations were detected by both methods. All mutation types detected by two methods are fully consistent. Conclu-sions Comparing with Sanger sequencing technology, xTAG liquidchip technology, which is able to detect mutations of exon 18~21 simultaneously, is more convenient and efficient for EGFR gene mutation detection in lung cancer.