中华病理学杂志
中華病理學雜誌
중화병이학잡지
Chinese Journal of Pathology
2014年
12期
827-830
,共4页
赵倩倩%梁茜子%韩洪秀
趙倩倩%樑茜子%韓洪秀
조천천%량천자%한홍수
子宫内膜异位症%神经纤维%受体,大麻酚,CB1
子宮內膜異位癥%神經纖維%受體,大痳酚,CB1
자궁내막이위증%신경섬유%수체,대마분,CB1
Endometriosis%Nerve Fibers%Receptor,cannabinoid,CB1
目的:探讨大麻素1型受体( CB1R)在子宫内膜异位囊肿组织神经生长中的作用。方法利用免疫荧光和免疫印迹方法检测CB1R激动剂、阻断剂对18只大鼠子宫内膜异位囊肿组织中泛神经标志物基因蛋白产物(PGP)9.5表达水平的影响。结果免疫荧光结果显示PGP 9.5在异位囊肿组织的神经纤维上表达,主要分布在囊肿门处。免疫印迹结果显示随着异位囊肿的生长,PGP 9.5(2周:0.38±0.05;4周:0.63±0.03;8周:0.80±0.07,P<0.01)和CB1R(2周:0.48±0.04;4周:0.68±0.01;8周:0.80±0.03,P<0.01)的蛋白表达水平均逐渐增加。另外,与对照组相比(0.75±0.01),鞘内注射CB1R激动剂 ACPA使囊肿组织内 PGP 9.5的蛋白表达增加(0.81±0.01,P <0.05),而CB1R阻断剂AM251使囊肿组织内PGP 9.5的蛋白表达下降(0.67±0.03,P <0.01)。结论大麻素CB1 R促进子宫内膜异位组织的神经生长。
目的:探討大痳素1型受體( CB1R)在子宮內膜異位囊腫組織神經生長中的作用。方法利用免疫熒光和免疫印跡方法檢測CB1R激動劑、阻斷劑對18隻大鼠子宮內膜異位囊腫組織中汎神經標誌物基因蛋白產物(PGP)9.5錶達水平的影響。結果免疫熒光結果顯示PGP 9.5在異位囊腫組織的神經纖維上錶達,主要分佈在囊腫門處。免疫印跡結果顯示隨著異位囊腫的生長,PGP 9.5(2週:0.38±0.05;4週:0.63±0.03;8週:0.80±0.07,P<0.01)和CB1R(2週:0.48±0.04;4週:0.68±0.01;8週:0.80±0.03,P<0.01)的蛋白錶達水平均逐漸增加。另外,與對照組相比(0.75±0.01),鞘內註射CB1R激動劑 ACPA使囊腫組織內 PGP 9.5的蛋白錶達增加(0.81±0.01,P <0.05),而CB1R阻斷劑AM251使囊腫組織內PGP 9.5的蛋白錶達下降(0.67±0.03,P <0.01)。結論大痳素CB1 R促進子宮內膜異位組織的神經生長。
목적:탐토대마소1형수체( CB1R)재자궁내막이위낭종조직신경생장중적작용。방법이용면역형광화면역인적방법검측CB1R격동제、조단제대18지대서자궁내막이위낭종조직중범신경표지물기인단백산물(PGP)9.5표체수평적영향。결과면역형광결과현시PGP 9.5재이위낭종조직적신경섬유상표체,주요분포재낭종문처。면역인적결과현시수착이위낭종적생장,PGP 9.5(2주:0.38±0.05;4주:0.63±0.03;8주:0.80±0.07,P<0.01)화CB1R(2주:0.48±0.04;4주:0.68±0.01;8주:0.80±0.03,P<0.01)적단백표체수평균축점증가。령외,여대조조상비(0.75±0.01),초내주사CB1R격동제 ACPA사낭종조직내 PGP 9.5적단백표체증가(0.81±0.01,P <0.05),이CB1R조단제AM251사낭종조직내PGP 9.5적단백표체하강(0.67±0.03,P <0.01)。결론대마소CB1 R촉진자궁내막이위조직적신경생장。
Objective To investigate whether cannabinoid receptor 1 (CB1R) is involved in nerve growth in endometriosis-associated ectopic cyst.Methods The effect of CB1R agonist and antagonist on the expression of pan-neuronal marker protein gene product ( PGP ) 9.5 in ectopic cyst was examined by immunofluorescence and Western blot in endometriosis model of 18 rats.Results Immunofluorescence revealed that PGP 9.5 was expressed in the nerve fibers and was mainly distributed in the cyst hilum. Western blot revealed that the protein density of either PGP 9.5 (2 week:0.38 ±0.05;4 week:0.63 ± 0.03;8 week:0.80 ±0.07, P<0.01) or CB1R(2 week:0.48 ±0.04;4 week:0.68 ±0.01;8 week:0.80 ±0.03, P<0.01) in the ectopic cyst increased with cyst size.In addition, compared to control group (0.75 ±0.01), PGP 9.5 expression in the ectopic cyst was promoted by CB1R agonist ACPA (0.81 ± 0.01, P <0.05), and inhibited by CB1R antagonist AM251 (0.67 ±0.03, P <0.01) .Conclusions CB1R was involved in the nerve growth of ectopic cyst associated with endometriosis.