CAJ | 학술논문

目的：研究杜仲及其盐制品对去卵巢大鼠骨质疏松症的治疗作用，并探讨盐制对其治疗作用的影响。方法40只SD雌性大鼠分为伪手术组、模型对照组、杜仲生品治疗组和杜仲盐制品治疗组，除假手术组施行假手术外，其余均行手术彻底摘除卵巢，术后4周开始杜仲生品及盐制品水提液灌胃给药（4．0 g／kg?d），实验过程称体质量，连续给药12周后，颈动脉取血，测定血钙（S－Ca）、血磷（S－P）含量，ELISA试剂盒双抗体夹心法测定血清碱性磷酸酶（ALP）、骨钙素（OCN）、雌二醇（E2）含量；动物处死后剥取完整子宫称重，采用HE染色法进行子宫病理学检查，剥离双侧股骨，双能X射线衍射法测定大鼠股骨骨密度。 HE染色法观察股骨骨小梁形态和成骨细胞数量，micro－CT扫描观察股骨骨小梁微体系结构。结果杜仲生品及盐制品能有效抑制去卵巢所致的大鼠体重增加，升高血清钙、雌二醇的含量，降低血磷、碱性磷酸酶和骨钙素的含量。增加大鼠股骨骨密度，改善股骨骨小梁微体系结构，增加股骨骨小梁成骨细胞数量，且长期用药对子宫无明显刺激作用。结论杜仲生品及盐制品对于大鼠去卵巢所引起的骨质疏松症有良好治疗作用，且盐制品效果优于生品。
목적：연구두중급기염제품대거란소대서골질소송증적치료작용，병탐토염제대기치료작용적영향。방법40지SD자성대서분위위수술조、모형대조조、두중생품치료조화두중염제품치료조，제가수술조시행가수술외，기여균행수술철저적제란소，술후4주개시두중생품급염제품수제액관위급약（4．0 g／kg?d），실험과정칭체질량，련속급약12주후，경동맥취혈，측정혈개（S－Ca）、혈린（S－P）함량，ELISA시제합쌍항체협심법측정혈청감성린산매（ALP）、골개소（OCN）、자이순（E2）함량；동물처사후박취완정자궁칭중，채용HE염색법진행자궁병이학검사，박리쌍측고골，쌍능X사선연사법측정대서고골골밀도。 HE염색법관찰고골골소량형태화성골세포수량，micro－CT소묘관찰고골골소량미체계결구。결과두중생품급염제품능유효억제거란소소치적대서체중증가，승고혈청개、자이순적함량，강저혈린、감성린산매화골개소적함량。증가대서고골골밀도，개선고골골소량미체계결구，증가고골골소량성골세포수량，차장기용약대자궁무명현자격작용。결론두중생품급염제품대우대서거란소소인기적골질소송증유량호치료작용，차염제품효과우우생품。
Objective To evaluate the anti-osteoporosis effect of Eucommia ulmoides Oliv.and the effect of salt-roast processing product on the treatment efficacy in ovariectomized rats.Methods Forty female SD rats were divided into Sham group, OVX group, Eucommia ulmoides Oliv.group, and salt-roast processing Eucommia ulmoides Oliv.group.All the rats except in Sham group were ovariectomized.After 4 weeks of the operation, rats received oral Eucommia ulmoides Oliv.or its salt-roast processing product (4.0 g/kg?d) daily.The body weight of the rats was recorded.After a 12-week treatment, the serum calcium (S-Ca) and phosphorus ( S-P) were measured.Serum ALP, osteocalcin ( OCN) , and estrogen ( E2 ) were determined using ELISA kits.The uterus was weighed after execution of the rats and pathological examination was performed using HE staining.Bone mineral density of the bilateral femur was tested using dual-energy X-ray absorptiometry.Morphology of the trabecular and the number of the osteoblast were observed using HE staining.Microarchitecture of the trabecular bone was observed using micro-CT scan.Results Oral administration of Eucommia ulmoides Oliv.and the salt-roast processing product effectively inhibited ovariectomy-induced the body weight increase, increased S-Ca and E2 , decreased S-P, ALP, and OCN.They increased BMD of the femur, improved microarchitecture of the trabecular bone, and increased the number the osteoblast.The long-term use of the drug had no stimulating effect on the uterus.Conclusion Eucommia ulmoides Oliv.and the salt-roast processing product have good therapeutic effect on ovariectomy-induced osteoporosis in rats, and the effect of salt-roast processing product is better than unroasted drug.