CAJ | 학술논문

目的：观察铁调素对小鼠单核细胞RAW264．7膜铁转运蛋白1（ FPN1）的表达，探讨铁调素对RAW264．7细胞作用的可能通路和机制。方法将不同浓度的铁调素加入含有核因子κB受体活化因子配体（ RANKL）的RAW264．7细胞培养基，24 h后用免疫荧光和Western－blot方法测定FPN1的表达，共聚焦显微镜（ CLSM ）测定细胞内铁离子的浓度。结果 RAW264．7细胞膜上存在FPN1受体的阳性表达；在本实验铁调素浓度干预范围内，FPN1的表达随着铁调素浓度的增加呈浓度依赖性降低（P＜0．05），同时细胞内铁离子的含量随着铁调素浓度的增加呈浓度依赖性增加（P＜0．05）。结论小鼠单核细胞RAW264．7是铁调素作用的靶细胞，铁调素可通过降解其细胞膜上的FPN1增加细胞内的铁离子。
목적：관찰철조소대소서단핵세포RAW264．7막철전운단백1（ FPN1）적표체，탐토철조소대RAW264．7세포작용적가능통로화궤제。방법장불동농도적철조소가입함유핵인자κB수체활화인자배체（ RANKL）적RAW264．7세포배양기，24 h후용면역형광화Western－blot방법측정FPN1적표체，공취초현미경（ CLSM ）측정세포내철리자적농도。결과 RAW264．7세포막상존재FPN1수체적양성표체；재본실험철조소농도간예범위내，FPN1적표체수착철조소농도적증가정농도의뢰성강저（P＜0．05），동시세포내철리자적함량수착철조소농도적증가정농도의뢰성증가（P＜0．05）。결론소서단핵세포RAW264．7시철조소작용적파세포，철조소가통과강해기세포막상적FPN1증가세포내적철리자。
Objective To investigate the effect of hepcidin on the expression of ferroportin 1 ( FPN1 ) in RAW264.7 cells in vitro, and to explore the possible mechanism.Methods Mouse monocytes RAW264.7 were treated with different concentrations of hepcidin in the presence of receptor activator of NF-Kb ligand ( RANKL) .After 24 hours, the expression of FPN1 was detected using Western blotting and immunofluorescence, respectively.The intracellular iron concentration was measured using a confocal laser scanning microscope.Results The results documented the existence of FPN1 at the membrane of RAW264.7 cells, and the expression of FPN1 was markedly downoregulated by hepcidin in a concentration-dependent manner ( p <0.05 ) .Furthermore, hepcidin increased intracellular iron in RAW264.7 cells in a concentration-dependent manner (p<0.05).Conclusion RAW264.7 cells are target cells of hepcidin.Hepcidin can increase intracellular iron by degradation of FPN1 at the membrane of RAW264.7 cells.