军事医学
軍事醫學
군사의학
BULLETIN OF THE ACADEMY OF MILITARY MEDICAL SCIENCES
2014年
12期
965-967,980
,共4页
段翠密%杨锡琴%修冰水%刘志强%张旭辉%戴振华%阙海萍%冯晓燕%张贺秋
段翠密%楊錫琴%脩冰水%劉誌彊%張旭輝%戴振華%闕海萍%馮曉燕%張賀鞦
단취밀%양석금%수빙수%류지강%장욱휘%대진화%궐해평%풍효연%장하추
乳腺肿瘤%抗原修复%人乳腺珠蛋白%免疫组织化学%石蜡%显微切片术
乳腺腫瘤%抗原脩複%人乳腺珠蛋白%免疫組織化學%石蠟%顯微切片術
유선종류%항원수복%인유선주단백%면역조직화학%석사%현미절편술
breast neoplasms%antigen retrieval%human mammglobin%immunohistochemistry%paraffin%microtomy
目的：探索一种简便有效的抗原修复方法，用于陈旧切片的人乳腺珠蛋白（human mammaglobin，hMAM）免疫组化检测。方法将乳腺癌患者陈旧乳腺组织石蜡切片脱蜡至水，按常规免疫组化步骤进行检测。其中，实验组直接将切片浸入pH 3．5的柠檬酸盐缓冲液中室温修复10～15 min；同时以微波和高压加热修复法处理相应患者石蜡切片作为对照，最后以SP检测系统检测抗原修复及与抗体反应的效果。结果实验组与高压组和微波组比较，修复效果较好，且无组织脱片与折损现象。结论建立了一种利用陈旧组织切片检测乳腺珠蛋白的简便、有效的抗原修复方法，并能很好地防止脱片现象发生。
目的：探索一種簡便有效的抗原脩複方法，用于陳舊切片的人乳腺珠蛋白（human mammaglobin，hMAM）免疫組化檢測。方法將乳腺癌患者陳舊乳腺組織石蠟切片脫蠟至水，按常規免疫組化步驟進行檢測。其中，實驗組直接將切片浸入pH 3．5的檸檬痠鹽緩遲液中室溫脩複10～15 min；同時以微波和高壓加熱脩複法處理相應患者石蠟切片作為對照，最後以SP檢測繫統檢測抗原脩複及與抗體反應的效果。結果實驗組與高壓組和微波組比較，脩複效果較好，且無組織脫片與摺損現象。結論建立瞭一種利用陳舊組織切片檢測乳腺珠蛋白的簡便、有效的抗原脩複方法，併能很好地防止脫片現象髮生。
목적：탐색일충간편유효적항원수복방법，용우진구절편적인유선주단백（human mammaglobin，hMAM）면역조화검측。방법장유선암환자진구유선조직석사절편탈사지수，안상규면역조화보취진행검측。기중，실험조직접장절편침입pH 3．5적저몽산염완충액중실온수복10～15 min；동시이미파화고압가열수복법처리상응환자석사절편작위대조，최후이SP검측계통검측항원수복급여항체반응적효과。결과실험조여고압조화미파조비교，수복효과교호，차무조직탈편여절손현상。결론건립료일충이용진구조직절편검측유선주단백적간편、유효적항원수복방법，병능흔호지방지탈편현상발생。
Objective To develop an antigen retrieval method for detection of human mammaglobin ( hMAM) immuno-histochemcal staining in old paraffin-embedded specimens .Methods The tissue sections in test group were put into dis-tilled water after deparaffinization and then moved into citric acid buffer ( pH 3.5) for 10-15 min.The other two meth-ods,microwave method and high pressure cooker method ,were compared as control groups at the same time .Finally, immu-nohistochemistry SP method was used to check the antibody in the sections .Results The color appearance in the test group (pH 3.5 citric solution) was better than that of microwave oven and high pressure cooker groups .In the test group, tissue sections were not easily cast off from the slices .Conclusion In this study,we have established a new and simple antigen retrieval method which will contribute to immunohistochemistry technology .
