CAJ | 학술논문

目的：研究盐度和温度调节副溶血弧菌（ Vibrio parahaemolyticus）的运动能力。方法将副溶血弧菌接种于不同盐度（0．5％、1．0％、2．0％和4．0％）的爬动和泳动平板上，37℃静置培养4．5 h后，通过比较不同盐度条件菌苔直径的差异来判定盐度对运动能力的影响。接种副溶血弧菌至含2．0％盐的爬动和泳动平板上，并分别置于37℃和26℃静止培养4．5 h后，通过比较不同温度下菌苔直径的差异来研究温度对运动能力的影响。结果与结论副溶血弧菌爬动不受盐度的影响，而其泳动与盐度呈正相关，2．0％时达到极值，4．0％时稍微下降；与26℃培养的结果相比，37℃培养时爬动和泳动均显著增强。这些结果表明盐度和温度能够调节副溶血弧菌的运动能力。
목적：연구염도화온도조절부용혈호균（ Vibrio parahaemolyticus）적운동능력。방법장부용혈호균접충우불동염도（0．5％、1．0％、2．0％화4．0％）적파동화영동평판상，37℃정치배양4．5 h후，통과비교불동염도조건균태직경적차이래판정염도대운동능력적영향。접충부용혈호균지함2．0％염적파동화영동평판상，병분별치우37℃화26℃정지배양4．5 h후，통과비교불동온도하균태직경적차이래연구온도대운동능력적영향。결과여결론부용혈호균파동불수염도적영향，이기영동여염도정정상관，2．0％시체도겁치，4．0％시초미하강；여26℃배양적결과상비，37℃배양시파동화영동균현저증강。저사결과표명염도화온도능구조절부용혈호균적운동능력。
Objective To investigate the effect of salinity and temperature on motility of Vibrio parahaemolyticus. Methods V.parahaemolyticus was inoculated on swarming or swimming agar plates containing different amounts of salinity (0.5%, 1.0%, 2.0%, and 4.0% NaCl, respectively), followed by incubation at 26 or 37℃, before the diameters of bacterial lawns were measured .Results and Conclusion The swarming motility was not affected by salinity , while the swimming motility was positively correlated with salinity .Maximum swimming occurred in 2.0% NaCl, and displayed a slight decline in salinity of 4.0%.Both swimming and swarming were affected by temperature , and the motility was signifi-cantly enhanced in 37℃vs 26℃.These results indicate that both salinity and temperature can modulate the motility of V. parahaemolyticus.