CAJ | 학술논문

目的：研究温肾生精饮（主要由鹿茸、人参、锁阳、大云、黄芪、淫羊藿和当归等15味药材组成）对环磷酰胺致小鼠睾丸氧化损伤的保护作用及其机制。方法将8周龄的昆明种雄性小鼠随机分组，正常对照组腹腔注射生理盐水，模型组、西药组和中药组连续5 d腹腔注射80 mg／（ kg? d）的环磷酰胺，然后将正常对照组和模型组小鼠灌胃生理盐水，西药组和中药组小鼠分别灌胃克罗米芬或温肾生精饮30 d。检测各组小鼠附睾精子密度和精子活率；观察睾丸生精上皮的发育；检测睾丸组织中丙二醛（MDA）、谷胱甘肽（GSH）、谷胱甘肽过氧化物酶（GPH－Px）和过氧化氢酶（CAT）的水平；免疫组织化学法检测睾丸生精小管中生存素（survivin）的表达；TUNEL法检测生精细胞的凋亡。结果与模型组和西药组比较，温肾生精饮显著提高了生精障碍小鼠的精子密度和精子活率，促进了生精小管上皮的发育，显著提高了生精障碍小鼠睾丸内GSH－Px和CAT的活性，降低了睾丸组织中MDA水平，且上调了生精细胞中生存素的表达水平，降低了生精小管和生精细胞的凋亡指数。结论温肾生精饮能显著修复环磷酰胺致小鼠睾丸生精功能损伤，其修复机制可能与提高睾丸组织中的抗氧化水平、减少生精细胞凋亡有关。
목적：연구온신생정음（주요유록용、인삼、쇄양、대운、황기、음양곽화당귀등15미약재조성）대배린선알치소서고환양화손상적보호작용급기궤제。방법장8주령적곤명충웅성소서수궤분조，정상대조조복강주사생리염수，모형조、서약조화중약조련속5 d복강주사80 mg／（ kg? d）적배린선알，연후장정상대조조화모형조소서관위생리염수，서약조화중약조소서분별관위극라미분혹온신생정음30 d。검측각조소서부고정자밀도화정자활솔；관찰고환생정상피적발육；검측고환조직중병이철（MDA）、곡광감태（GSH）、곡광감태과양화물매（GPH－Px）화과양화경매（CAT）적수평；면역조직화학법검측고환생정소관중생존소（survivin）적표체；TUNEL법검측생정세포적조망。결과여모형조화서약조비교，온신생정음현저제고료생정장애소서적정자밀도화정자활솔，촉진료생정소관상피적발육，현저제고료생정장애소서고환내GSH－Px화CAT적활성，강저료고환조직중MDA수평，차상조료생정세포중생존소적표체수평，강저료생정소관화생정세포적조망지수。결론온신생정음능현저수복배린선알치소서고환생정공능손상，기수복궤제가능여제고고환조직중적항양화수평、감소생정세포조망유관。
Objective To investigate the protective effect and the possible mechanism of Wenshen Shengjing Decoction (WSSJD)(including Cornu Cervi Nippon Parvum , Panax ginseng, Cynomorium songaricum, Cistanche deserticola,Radix Astragali, Epimedium brevicornum, Angelica sinensis.) on cyclophosphamide induced testicular oxidative damage in mice . Methods Eight-week-old male Kunming mice were assigned .Mice in normal control group received intraperitoneally nor-mal saline, and mice in Western medicine group ,WSSJD group and model group were injected intraperitoneally with cyclo-phosphamide [80 mg/(kg? d) ]for five days.Then mice in Western medicine group and WSSJD group were gavaged with clomifene citrate or WSSJD for 30 days consecutively .The epididymal sperm count and sperm viability were recorded , and the structure of seminiferous epithelium was observed .The content of malondialdehyde (MDA) and glutathione (GSH), and activities of glutathione peroxidase ( GSH-Px) and catalase ( CAT) in testicular tissue were detected .Immunohisto-chemical technique was used to detect the expression of survivin in spermatogenic cells .The apoptosis of spermatogenic cells in the testes was detected by TUNEL assay .Results Compared with model and Western medicine groups ,the epidid-ymal sperm count and sperm viability rate , the development of seminiferous epithelium , and the activities of GSH-Px and CAT significantly increased , while the MDA content significantly decreased in WSSJD group .The expression of survivin in spermatogenic cells was significantly improved .The apoptotic indexes of seminiferous tubules and spermatogenic cells significantly decreased in WSSJD group .Conclusion These results suggest that WSSJD can significantly improve the impaired reproductive function induced by cyclophospamide in mice by enhancing the antioxidative capability in testes and reducing the apoptosis of spermatogenic cells .