CAJ | 학술논문

目的：观察p16、cyclin D1在乳腺癌组织中的表达水平，并探讨其临床意义。方法采用免疫组织化学法对2010年2月至2013年4月武汉大学人民医院肿瘤中心手术切除的54例乳腺癌组织和乳腺癌旁正常组织及50例乳腺增生组织p16、cyclin D1的表达水平进行检测，探讨其与临床病理学指标间的关系。结果乳腺癌组织中p16蛋白阳性表达率为55．6％（30／54），而乳腺癌旁正常组织及乳腺增生组织中p16蛋白均为阳性表达；各组cyclin D1过度表达情况为：乳腺癌37．0％（20／54），乳腺癌旁正常组织20．4％（11／54），乳腺增生组织4．0％（2／50），乳腺癌组织中过度表达阳性率与其他组织的差异有统计学意义（P＜0．05）；p16蛋白表达在组织学分级的差异及cyclin D1表达在雌激素受体的差异均有统计学意义（P＜0．05）。结论乳腺癌组织中存在着cyclin D1的过度表达及p16蛋白的异常表达，p16、cyclin D1可成为判断乳腺癌组织分化程度和预后的实验室指标。
목적：관찰p16、cyclin D1재유선암조직중적표체수평，병탐토기림상의의。방법채용면역조직화학법대2010년2월지2013년4월무한대학인민의원종류중심수술절제적54례유선암조직화유선암방정상조직급50례유선증생조직p16、cyclin D1적표체수평진행검측，탐토기여림상병이학지표간적관계。결과유선암조직중p16단백양성표체솔위55．6％（30／54），이유선암방정상조직급유선증생조직중p16단백균위양성표체；각조cyclin D1과도표체정황위：유선암37．0％（20／54），유선암방정상조직20．4％（11／54），유선증생조직4．0％（2／50），유선암조직중과도표체양성솔여기타조직적차이유통계학의의（P＜0．05）；p16단백표체재조직학분급적차이급cyclin D1표체재자격소수체적차이균유통계학의의（P＜0．05）。결론유선암조직중존재착cyclin D1적과도표체급p16단백적이상표체，p16、cyclin D1가성위판단유선암조직분화정도화예후적실험실지표。
Objective To observe the expression levels of p16,cyclin D1 in breast cancer tissues,and explore its clinical significance.Methods The expression levels of p16,cyclin D1 in 54 cases of breast cancer tissues and adjacent normal tissues,50 cases of breast hyperplasia tissue resected in the Cancer Center of People′s Hospital Affiliated to Wuhan University from Feb .2010 to Apr.2013 were detected by immuno-histochemical method,and the relationship between the expression levels and clinical pathological indexes was explored.Results The positive expression rate of p16 protein in breast cancer tissues was 55.6% (30/54),and the expression of p16 protein in breast cancer adjacent normal and breast hyperplasia tissue were all positive.The over-expression of cyclinD1 protein of all groups were:breast cancer 37%(20/54),breast cancer adjacent normal tissues 20.4%(11/54),breast hyperplasia 4.0%(2/50),and there was statistically signifi-cant difference between the over-expression positive rate of breast cancer and other organizations(P<0.05). There were significant difference in the p16 protein expression on the histological grading(P <0.05), and there were significant differences of the cyclinD1 protein expression in the estrogen receptor (P<0.05).Con-clusion The is over-expression of cyclinD1 protein and abnormal expression of p16 protein in breast cancer tissues,which can be used as laboratory indexes of differentiation and prognosis of breast cancer.