CAJ | 학술논문

目的观察细胞因子诱导的自体DC、CIK、NK、αβT、γδT细胞治疗组织细胞肉瘤疗效。方法抽取患者自体外周血60~100 m L，采用血细胞分离机收集其单核细胞，经细胞因子诱导并扩增C I K、N K、αβT、γδT细胞及DC细胞；用流式细胞仪检测其表型后，静脉回输CIK、NK、αβT、γδT细胞，皮下注射DC细胞，观察组织细胞肉瘤变化。结果患者左乳肿块缩小＞50%，生活质量明显改善。结论细胞免疫治疗是一种化疗后组织细胞肉瘤有效的治疗手段，具有广阔的临床应用前景。
목적관찰세포인자유도적자체DC、CIK、NK、αβT、γδT세포치료조직세포육류료효。방법추취환자자체외주혈60~100 m L，채용혈세포분리궤수집기단핵세포，경세포인자유도병확증C I K、N K、αβT、γδT세포급DC세포；용류식세포의검측기표형후，정맥회수CIK、NK、αβT、γδT세포，피하주사DC세포，관찰조직세포육류변화。결과환자좌유종괴축소＞50%，생활질량명현개선。결론세포면역치료시일충화료후조직세포육류유효적치료수단，구유엄활적림상응용전경。
Objective To observe the clinical curative effect of cytokine induced autologous DC, CIK, NK,αβT andγδT cells to breast tissue cell sarcoma. Methods The mononuclear cells were separated from autologous peripheral blood in 60~100 mL by blood cell separator. The killer cells of CIK, NK,αβT,γδT and DC were induced and multiplied by cytokine in vitro. Flow cytometry was used to test their phenotype. CIK, NK,αβT,γδT cells were used by intravenous injection, and DC cells was used by subcutaneous injection. Results The tumor of the patient reduced above 50%, and his life quality was improved signiifcantly. Conclusion Cellular immunotherapy provides an effective approach for the treatment of tissue cell sarcoma after chemotherapy, which could provide new prospects in the clinical application.