中国医药导报
中國醫藥導報
중국의약도보
CHINA MEDICAL HERALD
2014年
35期
60-65
,共6页
刘玠%初李佳娃%张迪%曲海影%常越
劉玠%初李佳娃%張迪%麯海影%常越
류개%초리가왜%장적%곡해영%상월
抗宫炎凝胶%提取工艺%正交试验方法%广东紫珠%益母草%乌药
抗宮炎凝膠%提取工藝%正交試驗方法%廣東紫珠%益母草%烏藥
항궁염응효%제취공예%정교시험방법%엄동자주%익모초%오약
Kanggongyan Gels%Extraction process%Orthogonal experimental method%Guangdongzizhu%Yimucao%Wuyao
目的：优选七类新药抗宫炎凝胶剂的提取工艺。方法采用紫外可见分光光度法分别测定广东紫珠中的总黄酮、益母草中的盐酸水苏碱含量；采用高效液相色谱法对乌药中乌药醚内酯的含量进行测定。分别以总黄酮、盐酸水苏碱、乌药醚内酯的含量及干膏得率为考察指标，对处方中三味药进行提取工艺优化。利用正交试验设计法，以提取次数、提取时间、溶媒量、乙醇浓度为4个因素，每个因素设计3个水平。结果广东紫珠的最佳提取工艺为10倍量50%乙醇回流提取3次，每次3 h；益母草的最佳提取工艺为10倍量95%乙醇回流提取2次，每次1.5 h；乌药的最佳提取工艺为8倍量85%乙醇回流提取2次，每次1 h。结论优选的提取工艺科学合理，适于提取工艺中多因素的优选，可作为新药抗宫炎凝胶剂的工业化生产提取工艺，为制备安全稳定、速释高效新剂型的研究奠定科学基础。
目的：優選七類新藥抗宮炎凝膠劑的提取工藝。方法採用紫外可見分光光度法分彆測定廣東紫珠中的總黃酮、益母草中的鹽痠水囌堿含量；採用高效液相色譜法對烏藥中烏藥醚內酯的含量進行測定。分彆以總黃酮、鹽痠水囌堿、烏藥醚內酯的含量及榦膏得率為攷察指標，對處方中三味藥進行提取工藝優化。利用正交試驗設計法，以提取次數、提取時間、溶媒量、乙醇濃度為4箇因素，每箇因素設計3箇水平。結果廣東紫珠的最佳提取工藝為10倍量50%乙醇迴流提取3次，每次3 h；益母草的最佳提取工藝為10倍量95%乙醇迴流提取2次，每次1.5 h；烏藥的最佳提取工藝為8倍量85%乙醇迴流提取2次，每次1 h。結論優選的提取工藝科學閤理，適于提取工藝中多因素的優選，可作為新藥抗宮炎凝膠劑的工業化生產提取工藝，為製備安全穩定、速釋高效新劑型的研究奠定科學基礎。
목적：우선칠류신약항궁염응효제적제취공예。방법채용자외가견분광광도법분별측정엄동자주중적총황동、익모초중적염산수소감함량；채용고효액상색보법대오약중오약미내지적함량진행측정。분별이총황동、염산수소감、오약미내지적함량급간고득솔위고찰지표，대처방중삼미약진행제취공예우화。이용정교시험설계법，이제취차수、제취시간、용매량、을순농도위4개인소，매개인소설계3개수평。결과엄동자주적최가제취공예위10배량50%을순회류제취3차，매차3 h；익모초적최가제취공예위10배량95%을순회류제취2차，매차1.5 h；오약적최가제취공예위8배량85%을순회류제취2차，매차1 h。결론우선적제취공예과학합리，괄우제취공예중다인소적우선，가작위신약항궁염응효제적공업화생산제취공예，위제비안전은정、속석고효신제형적연구전정과학기출。
Objective To optimize the extraction process of the seventh drug for Kanggongyan Gels. Methods UV-visi-ble spectrophotometry was used to determine the contents of total flavonoids in Guangdongzizhu and stachydrine hy-drochloride in Yimucao; HPLC was adopted to determine the contents of lindera ether in Wuyao. The contents of total flavonoids, stachydrine hydrochloride, lindera ether ester as well were taken as extract rate as assessment index to opti-mize the extraction process of the shamisen drugs in prescription. By using orthogonal experimental design method, the extraction times, extraction time, solvent usage and the concentration of ethanol as factors were taken, and three levels of each factor were designed. Results The best program for extraction of Guangdongzizhu was 10 times of 50% ethanol extraction in 3 h each for 3 times; the best program for extraction of Yimucao was 10 times of 95% ethanol extraction in 1.5 h each for 2 times; the best program for extraction of Wuyao was 8 times of 85% ethanol extraction in 1 h each for 2 times. Conclusion The optimal extraction technology is reasonable and scientific, and it can be used for optimiz-ing factors in extraction technology and industrial production. For researching the preparation of security and stability, immediate-release dosage form of efficient lays the scientific foundation.
