CAJ | 학술논문

目的：优选肺结核诊断中理想的痰TB-DNA提取方法。方法选择我院肺科门诊肺结核患者185例，提取其痰液样本，应用煮沸裂解法、酚/氯仿法、以及进口离心柱法进行分析，对比实时荧光PCR结果。结果进口离心柱法下TB-DNA阳性检出率为81.08%（150/185），明显高于煮沸裂解法以及酚/氯仿法，P＜0.05。结论在对疑似肺结核患者痰液标本进行检测中，可优先选择进口离心柱法作为TB-DNA的有效提取方法。
목적：우선폐결핵진단중이상적담TB-DNA제취방법。방법선택아원폐과문진폐결핵환자185례，제취기담액양본，응용자비렬해법、분/록방법、이급진구리심주법진행분석，대비실시형광PCR결과。결과진구리심주법하TB-DNA양성검출솔위81.08%（150/185），명현고우자비렬해법이급분/록방법，P＜0.05。결론재대의사폐결핵환자담액표본진행검측중，가우선선택진구리심주법작위TB-DNA적유효제취방법。
Objective?To?select?ideal?in?tuberculosis?diagnosis?sputum?TB-DNA?extraction?method.?Method?185?cases?with?pulmonary?outpatient?tuberculosis?patients?choose?our?hospital,?extract?sputum?samples,?the?application?of?boiling?pyrolysis?method,?phenol/chloroform?method,?column?and?import?the centrifugal method were analyzed, and compared the real-time lfuorescent PCR results. Results?Under?import?centrifugal?column?method?TB-DNA?positive detection rate was 81.08%(150/185), signiifcantly higher than the boiling cracking method and phenol/chloroform method, P<0.05.?Conclusions?In?patients?with?suspected?pulmonary?tuberculosis?in?sputum?specimens?for?testing,?can?choose?imported?centrifugal?column?priority?method?as?effective?TB-DNA?extraction?method.