CAJ | 학술논문

目的：建立0～14岁长春市汉族儿童血清碱性磷酸酶（ALP）的参考区间。方法采用日立7600-210全自动生化分析仪检测4211名健康体检儿童（男2090名，女2121名）血清 ALP。弃离群值后，判断数据是否正态分布。One-Way ANOVA 比较组间差异及确定是否需性别、年龄分组。非参数方法计算参考值的2.5百分位数和97.5百分位数，R 语言计算90%置信区间。结果长春市儿童 ALP 参考区间有年龄及性别差异。婴儿出生1个月内 ALP 水平较低，此后开始增高，1个月至11岁儿童 ALP 水平相对稳定且无性别差异。12岁后儿童 ALP 水平逐渐出现性别差异，12～14岁女孩 ALP 水平逐渐下降，而12～14岁男孩 ALP 水平高于同年龄段女孩。年龄、性别合并后的参考区间包括0～30 d、1～12月、1～10岁、11岁、12～14岁（男）、12岁（女）、13岁（女）及14岁（女）。结论建立儿童年龄、性别相关的血清 ALP 参考区间对儿童预防保健及疾病状况分析具有临床应用价值。
목적：건립0～14세장춘시한족인동혈청감성린산매（ALP）적삼고구간。방법채용일립7600-210전자동생화분석의검측4211명건강체검인동（남2090명，녀2121명）혈청 ALP。기리군치후，판단수거시부정태분포。One-Way ANOVA 비교조간차이급학정시부수성별、년령분조。비삼수방법계산삼고치적2.5백분위수화97.5백분위수，R 어언계산90%치신구간。결과장춘시인동 ALP 삼고구간유년령급성별차이。영인출생1개월내 ALP 수평교저，차후개시증고，1개월지11세인동 ALP 수평상대은정차무성별차이。12세후인동 ALP 수평축점출현성별차이，12～14세녀해 ALP 수평축점하강，이12～14세남해 ALP 수평고우동년령단녀해。년령、성별합병후적삼고구간포괄0～30 d、1～12월、1～10세、11세、12～14세（남）、12세（녀）、13세（녀）급14세（녀）。결론건립인동년령、성별상관적혈청 ALP 삼고구간대인동예방보건급질병상황분석구유림상응용개치。
Objective To establish pediatric reference intervals of serum alkaline phosphatase (ALP) for healthy children with an age range of 0-14 years old in Changchun. Methods A total of 4 211 healthy children (2 090 males and 2 121 females) were enrolled in Changchun. ALP was performed on Hitachi 7600-210 automatic biochemical analyzer. After outlier data exclusion, data were estimated to or not to follow Gaussian distributions. One-way ANOVA was used to compare the differences of gender and age groups. The 2.5th and 97.5th percentiles of ALP were calculated by nonparametric method and 90%confidence intervals were computed by R language. Results The study showed there were apparent age and gender variations of the reference intervals for ALP. After a temporary low level in newborns, there was an increase from 1 month. The reference intervals of ALP were relatively stable and there was no significant gender dependence from 1 month to 11 years old. The dependency of ALP reference intervals on age was different for boys and girls after 12 years old. For girls aged 12 to 14 years old there was a significant decrease for ALP level. However, the ALP level of boys with an age range of 12-14 years old was higher than relative girls. Combined reference interval of ALP include 0-30 days, 1-12 months, 1-10 years old, 11 years old, 12-14 years old (boys), 12 years old (girls), 13 years old (girls) and 14 years old (girls). Conclusion Establishment of serum ALP reference interval is important for prevention, health care and diseases evaluation of children.