CAJ | 학술논문

蒙药嘎日迪－13味丸对脑缺血大鼠海马组织白介素－1β、肿瘤坏死因子－α蛋白及神经细胞凋亡的影响
몽약알일적－13미환대뇌결혈대서해마조직백개소－1β、종류배사인자－α단백급신경세포조망적영향
Effect of Mongolian medicine Garidi-13 pill on IL-1β, TNF-αprotein and nerve cell apoptosis of cerebral ischemia hippocampus tis-sue of rats

万方数据

中国老年学杂志中國老年學雜誌 중국노년학잡지
CHINESE JOURNAL OF GERONTOLOGY
2015年 1期 152-154 ,共3页

陶春%林琳%宋葆华%汤文莉%宝鲁尔%宣丽颖%翟景波%刘鑫%张冬丽%霍万学%刘月英

도춘%림림%송보화%탕문리%보로이%선려영%적경파%류흠%장동려%곽만학%류월영

嘎日迪-13 味丸%脑缺血%细胞凋亡%炎性因子嘎日迪-13 味汍%腦缺血%細胞凋亡%炎性因子
알일적-13 미환%뇌결혈%세포조망%염성인자
Garidi -13 pill%Cerebral ischemia%Cell apoptosis%Inflammatory factor

目的：探讨蒙药嘎日迪－13味丸对脑缺血大鼠海马组织炎性细胞因子白介素－1β（ IL－1β）和肿瘤坏死因子－α（ TNF－α）表达及神经细胞凋亡的影响。方法采用枕大池二次注血法至大鼠脑组织缺血损伤模型，在各时间段断头取脑，用 ELISA 法和流式细胞仪分别检测脑缺血海马组织中 IL－1β和 TNF－α的含量及细胞凋亡数量的百分比。结果与正常组比较，模型组海马组织中 IL－1β和 TNF－α升高，细胞凋亡百分比增多（ P＜0．05）；与模型组比较，嘎日迪－13味丸组大鼠海马组织中 IL－1β和 TNF－α含量降低，细胞凋亡百分比数减少（ P＜0．05）。结论嘎日迪－13味丸能降低大鼠脑缺血组织神经细胞凋亡数量，减轻缺血损伤程度，其机制可能与降低脑组织内 IL－1β和 TNF－α含量有关。
목적：탐토몽약알일적－13미환대뇌결혈대서해마조직염성세포인자백개소－1β（ IL－1β）화종류배사인자－α（ TNF－α）표체급신경세포조망적영향。방법채용침대지이차주혈법지대서뇌조직결혈손상모형，재각시간단단두취뇌，용 ELISA 법화류식세포의분별검측뇌결혈해마조직중 IL－1β화 TNF－α적함량급세포조망수량적백분비。결과여정상조비교，모형조해마조직중 IL－1β화 TNF－α승고，세포조망백분비증다（ P＜0．05）；여모형조비교，알일적－13미환조대서해마조직중 IL－1β화 TNF－α함량강저，세포조망백분비수감소（ P＜0．05）。결론알일적－13미환능강저대서뇌결혈조직신경세포조망수량，감경결혈손상정도，기궤제가능여강저뇌조직내 IL－1β화 TNF－α함량유관。
Objective To investigate the effects of Mongolian medicine Garidi -13 pill on inflammatory cytokines interleukin-1β( IL-β) and tumor necrosis factor-α(TNF-α) expression and nerve cell apoptosis of cerebral ischemia hippocampus tissue of rats .Methods Two times of blood injection to the cistema magna was used to make the rat model of cerebral ischemia .Brain tissue was acquired at each time point.Flow cytometry and ELISA were used to detect the number of apoptotic cells , IL-1βand TNF-αcontent in hippocampus tissue.Re-sults Compared with normal group, IL-1βand TNF-αin hippocampus were increased , apoptosis percentage number was increased of model group (P<0.05).Conclusions Garidi-13 can reduce the number of apoptosis nerve cell in ischemia brain tissue and reduce the severity of ischemic injury, its mechanism may be related to reduce IL -1βand TNF-αcontent of brain.