CAJ | 학술논문

目的：观察四神丸对溃疡性结肠炎（UC）大鼠白细胞介素（IL）－1β、－4肿瘤坏死因子（TNF）－α的影响。方法 Wistar 大鼠40只分为四组，采用2、4、6－三硝基苯磺酸（TNBS）乙醇灌肠法造模，光镜观察结肠组织病理形态并评分，采用酶联免疫法（ ELISA）检测大鼠血清 IL－1β、 TNF－α、IL－4的水平。结果模型组大鼠结肠组织黏膜层可见溃疡形成，且有明显的炎症、充血存在，证实造模成功。模型组大鼠血清 IL－1β、TNF－α含量均高于空白组（P＜0．05），而 IL－4含量低于空白组（P＜0．01）；治疗后，四神丸组血清 IL－1β、TNF－α水平均较模型组降低（P＜0．05、P＜0．01），而 IL－4含量却显著升高（P＜0．01）。结论四神丸能够通过下调 IL－1β、TNF－α的表达，升高 IL－4的表达，调节肠道异常免疫反应、抑制炎症反应，修复溃疡，进而治疗 UC。
목적：관찰사신환대궤양성결장염（UC）대서백세포개소（IL）－1β、－4종류배사인자（TNF）－α적영향。방법 Wistar 대서40지분위사조，채용2、4、6－삼초기분광산（TNBS）을순관장법조모，광경관찰결장조직병리형태병평분，채용매련면역법（ ELISA）검측대서혈청 IL－1β、 TNF－α、IL－4적수평。결과모형조대서결장조직점막층가견궤양형성，차유명현적염증、충혈존재，증실조모성공。모형조대서혈청 IL－1β、TNF－α함량균고우공백조（P＜0．05），이 IL－4함량저우공백조（P＜0．01）；치료후，사신환조혈청 IL－1β、TNF－α수평균교모형조강저（P＜0．05、P＜0．01），이 IL－4함량각현저승고（P＜0．01）。결론사신환능구통과하조 IL－1β、TNF－α적표체，승고 IL－4적표체，조절장도이상면역반응、억제염증반응，수복궤양，진이치료 UC。
Objective To observe the influence of the Si Shen Pill on interleukin -1β(IL-1β) ,TNF-αand interleukin-4(IL-4)in ulcerative colitis (UC) rats.Methods Experimental rats were divided into four groups .Model rats were made by means of the TNBS /ethanol enema.Colon organization pathological changes were observed by the light microscope .Serum IL-1β,TNF-αand IL-4 were detected by ELISA.Results Ulceration,inflammation,congestion existed in colon mucosa of model rats ,which meant the models were made successful -ly.IL-1βand TNF-αof model rats were higher and IL-4 was lower than those of rats in blank group (P<0.05,P<0.01).After treatment, compared with the model group,IL-1βand TNF-αwere reduced and IL-4 was increased in the Si Shen Pill group (P<0.05,P<0.01).Con-clusions Si Shen Pill can reduce the IL-1βand TNF-α,increase IL-4,regulate immune abnormalities in intestines ,inhibit inflammation,re-pair ulcers and cure UC.