CAJ | 학술논문

目的：探讨吸入麻醉药异氟烷对转 APP 基因小鼠学习记忆功能的影响及海藻糖的干预保护作用。方法60只12月龄转 APP 小鼠随机分为空白对照组（Control 组）、异氟烷组（Iso 组）、海藻糖组（Iso＋Tre 组），每组20只。 Iso＋Tre 组和 Iso 组分别于麻醉前30 min 腹腔注射海藻糖400μg ／kg 或等量生理盐水，然后给予1．4％异氟烷吸入麻醉2 h，麻醉后24 h 应用 Morris 水迷宫测试小鼠学习记忆能力，麻醉后6、24 h 杀鼠取脑，采用 TUNEL 染色观察各组小鼠海马区神经元凋亡，应用 ELISA 法检测 Aβ含量，Western 印迹法检测 Bcl－2、Bax、Caspase－3的表达变化。结果与 Con－trol 组相比，Iso 组小鼠总体的平均逃避潜伏期明显延长，空间探索时间明显缩短（ P ＜0．01），海马神经元凋亡指数、Aβ含量、Bax／Bcl－2比值及Caspase－3表达均明显增加（P＜0．01）；Iso＋Tre 组与 Iso 组相比，逃避潜伏期缩短，空间探索时间延长（P＜0．05），Aβ含量、Bax ／Bcl－2比值及 Caspase－3表达均明显减少（P＜0．01）。结论海藻糖能够改善异氟烷诱导转 APP 基因小鼠学习记忆能力的损害，减少海马神经元的凋亡及 Aβ的生成。
목적：탐토흡입마취약이불완대전 APP 기인소서학습기억공능적영향급해조당적간예보호작용。방법60지12월령전 APP 소서수궤분위공백대조조（Control 조）、이불완조（Iso 조）、해조당조（Iso＋Tre 조），매조20지。 Iso＋Tre 조화 Iso 조분별우마취전30 min 복강주사해조당400μg ／kg 혹등량생리염수，연후급여1．4％이불완흡입마취2 h，마취후24 h 응용 Morris 수미궁측시소서학습기억능력，마취후6、24 h 살서취뇌，채용 TUNEL 염색관찰각조소서해마구신경원조망，응용 ELISA 법검측 Aβ함량，Western 인적법검측 Bcl－2、Bax、Caspase－3적표체변화。결과여 Con－trol 조상비，Iso 조소서총체적평균도피잠복기명현연장，공간탐색시간명현축단（ P ＜0．01），해마신경원조망지수、Aβ함량、Bax／Bcl－2비치급Caspase－3표체균명현증가（P＜0．01）；Iso＋Tre 조여 Iso 조상비，도피잠복기축단，공간탐색시간연장（P＜0．05），Aβ함량、Bax ／Bcl－2비치급 Caspase－3표체균명현감소（P＜0．01）。결론해조당능구개선이불완유도전 APP 기인소서학습기억능력적손해，감소해마신경원적조망급 Aβ적생성。
Objective To observe the influence of inhalation anesthestic isoflurane on the learning -memory ability of APP transgenic mice and protective effects of trehalose .Methods 60 APP transgenic mice at the age of 12 months were divided into control , Iso and Iso+Tre groups.The abilities of learning and memory were investigated by Morris water maze .TUNEL assay was employed to observe the hipp -ocampal neuron apoptosis of the mice in each group .The amount of Aβwas checked by ELISA assay kit and the variations of Bcl -2, Bax, Caspase-3 were examined by Western blot.Results In comparison with those of control group , the mean escape latency of Iso group was prolonged, the spatial exploring time was reduced , apoptotic index, the amount of Aβ, the ratio of Bax/Bcl-2, and the expression of Caspase-3 were increased.Moreover, compared to those of Iso group, escape latency of Iso +Tre was shorter, longer spatial exploring time was longer, the amount of Aβ, ratio of Bax /Bcl-2, and Caspase-3 expression were lower.Conclusions Trehalose could improve the recover -y of the learning and memory of APP transgenic mice after isoflurane anesthesia , the hippocampal neuron apoptosis and the production of A βcan be inhibited by trehalose.