CAJ | 학술논문

目的：探讨香烟烟雾提取物（CSE）对Ⅱ型肺泡上皮细胞中 Derlin－1蛋白及其 mRNA 表达的影响。方法体外培养Ⅱ型肺泡上皮细胞（A549），加入10％浓度的 CES 作用于细胞0、3、6、12、24、48 h，采用免疫蛋白印迹（WB）检测 Derlin－1蛋白表达水平的变化，并运用逆转录聚合酶链反应（RT－PCR）检测 Derlin－1 mRNA 含量的变化。结果10％浓度的 CSE 作用下，Derlin－1蛋白的表达逐渐提高，并在6 h 达最高，随后逐渐下降， Derlin－1 mRNA 含量变化与 Derlin－1蛋白表达一致。结论香烟烟雾可影响Ⅱ型肺泡上皮细胞中 Derlin－1表达的变化，可能与 Derlin－1是内质网应激诱导凋亡的抑制剂有关。
목적：탐토향연연무제취물（CSE）대Ⅱ형폐포상피세포중 Derlin－1단백급기 mRNA 표체적영향。방법체외배양Ⅱ형폐포상피세포（A549），가입10％농도적 CES 작용우세포0、3、6、12、24、48 h，채용면역단백인적（WB）검측 Derlin－1단백표체수평적변화，병운용역전록취합매련반응（RT－PCR）검측 Derlin－1 mRNA 함량적변화。결과10％농도적 CSE 작용하，Derlin－1단백적표체축점제고，병재6 h 체최고，수후축점하강， Derlin－1 mRNA 함량변화여 Derlin－1단백표체일치。결론향연연무가영향Ⅱ형폐포상피세포중 Derlin－1표체적변화，가능여 Derlin－1시내질망응격유도조망적억제제유관。
Objective To explore the effect of cigarette smoke extract (CSE) on expressional levels of Derlin-1 protein and mRNA in the cultured A549 cells those were rat alveolar type Ⅱ cells lines.Methods Western blot was used to detect the level of Derlin -1 protein expression exposured to 10% concentration of CSE at different time (0,3,6,12,24,48 h).RT-PCR was used to measure the change of Der -lin-1 mRNA.Results In 10% concentration of CSE, Derlin-1 protein expression gradually was increased and reached the highest at 6 h and then decreased gradually.RT-PCR results consistented with the result of Western blot .Conclusions Cigarette smoke can affect the changes of the expression of Derlin-1 in alveolar typeⅡ cells, which may be associated with that Derlin -1 is the apoptosis inhibitor of endoplasmic re -ticulum stress.