CAJ | 학술논문

目的：研究抗代谢类抗肿瘤药物对人肿瘤细胞核苷酸代谢的影响。方法采用离子对高效液相色谱法测定药物作用前后12种人肿瘤细胞中的12种核苷酸组分的含量，通过对主成分分析并结合单因素方差分析及受试者工作曲线分析、寻找与药物作用相关的标记物。结果对照组与加药组之间核苷酸代谢模式存在差异，筛选出贡献较大的组分为ATP、ADP、GMP和UDP，结合ROC曲线分析可知ATP、GMP和UDP具有一定的诊断准确性。结论ATP、UDP和GMP为药物作用相关的最佳生物标记物。
목적：연구항대사류항종류약물대인종류세포핵감산대사적영향。방법채용리자대고효액상색보법측정약물작용전후12충인종류세포중적12충핵감산조분적함량，통과대주성분분석병결합단인소방차분석급수시자공작곡선분석、심조여약물작용상관적표기물。결과대조조여가약조지간핵감산대사모식존재차이，사선출공헌교대적조분위ATP、ADP、GMP화UDP，결합ROC곡선분석가지ATP、GMP화UDP구유일정적진단준학성。결론ATP、UDP화GMP위약물작용상관적최가생물표기물。
Objective To study the effects of antimetabolite agents on the nucleotide pools in human tumor cells .Methods Contents of twelve nucleotides in twelve human tumor cells of control group and drug‐treated group were determined by ion‐pair HPLC method .Several data processing methods were combined for the identification of potential biomarkers associated with the action of drugs ,including principal component analysis (PCA) ,one‐way analysis of variance significant test (ANOVA) ,and receiver oper‐ating characteristic (ROC) curve .Results A significant difference has been found in nucleotides between control group and drug‐treated group .The dominant metabolites were ATP ,ADP ,GMP and UDP ,which were further validated by ROC curve analysis . Conclusion ATP ,GMP and UDP were recognized as the best potential biomarkers associated with the drug action .