CAJ | 학술논문

目的：探讨氨基丁酸联合卡泊芬净抗白色假丝酵母菌生物被膜的协同作用。方法利用白色假丝酵母菌标准菌株SC5314，采用生物被膜形成实验，分为空白对照组、氨基丁酸单用组、卡泊芬净单用组、氨基丁酸联合卡泊芬净组，对比各组生物被膜形成情况。采用XT T还原法测定氨基丁酸、卡泊芬净单用以及氨基丁酸联合卡泊芬净对成熟生物被膜细胞代谢活性的抑制作用。采用YNB培养基菌丝形成实验，考察氨基丁酸与卡泊芬净合用是否具有协同抑制菌丝形成的作用。结果卡泊芬净0．1μg · mL －1联合氨基丁酸0．1μmol · L －1对白色假丝酵母菌SC5314生物被膜的形成具有显著的抑制作用。此外，XT T还原法测定氨基丁酸6．25μmol · L －1联合卡泊芬净0．1μg · mL －1时降低被膜细胞代谢活性的效率能够达到约15％。采用YNB培养基形成菌丝，氨基丁酸6．25μmol · L －1联合卡泊芬净0．1μg · mL －1对白色假丝酵母菌SC5314菌丝形成能力有显著的抑制作用。结论氨基丁酸联合卡泊芬净表现出显著的体外协同抗白色假丝酵母菌标准菌株SC5314生物被膜作用。
목적：탐토안기정산연합잡박분정항백색가사효모균생물피막적협동작용。방법이용백색가사효모균표준균주SC5314，채용생물피막형성실험，분위공백대조조、안기정산단용조、잡박분정단용조、안기정산연합잡박분정조，대비각조생물피막형성정황。채용XT T환원법측정안기정산、잡박분정단용이급안기정산연합잡박분정대성숙생물피막세포대사활성적억제작용。채용YNB배양기균사형성실험，고찰안기정산여잡박분정합용시부구유협동억제균사형성적작용。결과잡박분정0．1μg · mL －1연합안기정산0．1μmol · L －1대백색가사효모균SC5314생물피막적형성구유현저적억제작용。차외，XT T환원법측정안기정산6．25μmol · L －1연합잡박분정0．1μg · mL －1시강저피막세포대사활성적효솔능구체도약15％。채용YNB배양기형성균사，안기정산6．25μmol · L －1연합잡박분정0．1μg · mL －1대백색가사효모균SC5314균사형성능력유현저적억제작용。결론안기정산연합잡박분정표현출현저적체외협동항백색가사효모균표준균주SC5314생물피막작용。
Objective To investigate the enhanced effect of aminobutyric acid (GABA) combined with caspofungin on biofilm forma‐tion of Candida albicans .Methods Standard stains of Candida albicans SC5314 were used in this study .The group of caspofun‐gin with GABA comparing with the group of GABA or caspofungin alone were evaluated by observing the prevention of biofilm formation .Candida albicans SC5314 were cultured in YNB medium to induce the formation of hyphae and the metabolic activity was determined by XTT reduction assay .Results The group of caspofungin (0 .1μg · mL -1 ) showed no inhibition against biofilm formation of Candida albicans ,but caspofungin (0 .1 μg · mL -1 ) with GABA (0 .1 μmol · L -1 ) showed apparent inhibition a‐gainst biofilm formation of Candida albicans .The metabolic activity of caspofungin 0 .1 μg · mL -1 with GABA 6 .25 μmol · L -1 reduced to about 15% .Conclusion The combination of caspogungin with GABA possessed an enhanced effect against the biofilm formation of Candiada albicans SC5314 in vitro .