西北药学杂志
西北藥學雜誌
서북약학잡지
2015年
1期
28-30
,共3页
邱远金%朱国强%贾晓光%陈书强%李忠民
邱遠金%硃國彊%賈曉光%陳書彊%李忠民
구원금%주국강%가효광%진서강%리충민
四味抗痨丸%巴豆苷%高效液相色谱法%结核病
四味抗癆汍%巴豆苷%高效液相色譜法%結覈病
사미항로환%파두감%고효액상색보법%결핵병
Siweikanglao Pills%crotonside%HPLC%anti-tuberculosis
目的:建立高效液相色谱法测定四味抗痨丸中巴豆苷含量。方法采用高效液相色谱法测定四味抗痨丸中巴豆苷的含量。色谱柱为Luna C18柱,流动相为乙腈‐甲醇‐水(1∶4∶95),检测波长为292 nm ,流速为1.0 mL · min-1。结果巴豆苷在6.195×10-2~1.239μg范围内与峰面积有良好的线性关系,r=0.9999(n=7),平均回收率为98.4%,RSD为1.0%,经测定供试品中巴豆苷平均含量为0.2073mg·g -1。结论该方法简便、准确、重复性好,可用于四味抗痨丸中巴豆苷含量测定。
目的:建立高效液相色譜法測定四味抗癆汍中巴豆苷含量。方法採用高效液相色譜法測定四味抗癆汍中巴豆苷的含量。色譜柱為Luna C18柱,流動相為乙腈‐甲醇‐水(1∶4∶95),檢測波長為292 nm ,流速為1.0 mL · min-1。結果巴豆苷在6.195×10-2~1.239μg範圍內與峰麵積有良好的線性關繫,r=0.9999(n=7),平均迴收率為98.4%,RSD為1.0%,經測定供試品中巴豆苷平均含量為0.2073mg·g -1。結論該方法簡便、準確、重複性好,可用于四味抗癆汍中巴豆苷含量測定。
목적:건립고효액상색보법측정사미항로환중파두감함량。방법채용고효액상색보법측정사미항로환중파두감적함량。색보주위Luna C18주,류동상위을정‐갑순‐수(1∶4∶95),검측파장위292 nm ,류속위1.0 mL · min-1。결과파두감재6.195×10-2~1.239μg범위내여봉면적유량호적선성관계,r=0.9999(n=7),평균회수솔위98.4%,RSD위1.0%,경측정공시품중파두감평균함량위0.2073mg·g -1。결론해방법간편、준학、중복성호,가용우사미항로환중파두감함량측정。
Objective To establish an HPLC method for the determination of crotonside in Siweikanglao Pills .Methods HPLC anal‐ysis was carried out on a Luna C18 column and a mixture of acetonitrile‐methanol‐water (1∶4∶95) was used as the mobile phase , with the detection wavelength at 292 nm ,and the flow rate at 1 .0 mL · min-1 .Results The linear range of crotonside was from 6 .195 × 10-2 to 1 .239 μg .The correlation coefficient of the calibration curves was 0 .999 9 .The average recovery rate was 98 .4%with relative standard deviation of 1 .0% .The average content in Siweikanglao Pills was 0 .207 3 mg · g -1 .Conclusion The re‐sults showed that the method is simple ,accurate and repeatable ,and it is suitable for the quality control of Siweikanglao Pills .