CAJ | 학술논문

目的：建立青龙衣水解单宁含量测定的方法。方法以没食子酸为对照品，研究青龙衣水解单宁的含量测定，采用紫外‐可见分光光度法，确定改良的Folin‐Ciocalteu法运用于青龙衣单宁含量测定显色的最佳条件，测定青龙衣酸解前后总单宁的含量。结果测定没食子酸的标准曲线方程为：Y＝151．8 X＋0．041，在0．992～4．960μg · m L －1范围内线性关系良好，r＝0．999（n＝6）。优化的显色方法为1．0 mL的样品液，Folin‐Ciocalteu 1．5 mL ，加去离子水10 mL ，用70 g · L －1的碳酸钠定容至25 mL，30℃显色1．5h。结论该显色方法操作简便，准确度高、稳定性和重复性良好，既适用于未酸解单宁样品的含量测定，也适用于酸解后单宁样品的含量测定，对水解单宁类成分的含量测定具有普遍适用性。
목적：건립청룡의수해단저함량측정적방법。방법이몰식자산위대조품，연구청룡의수해단저적함량측정，채용자외‐가견분광광도법，학정개량적Folin‐Ciocalteu법운용우청룡의단저함량측정현색적최가조건，측정청룡의산해전후총단저적함량。결과측정몰식자산적표준곡선방정위：Y＝151．8 X＋0．041，재0．992～4．960μg · m L －1범위내선성관계량호，r＝0．999（n＝6）。우화적현색방법위1．0 mL적양품액，Folin‐Ciocalteu 1．5 mL ，가거리자수10 mL ，용70 g · L －1적탄산납정용지25 mL，30℃현색1．5h。결론해현색방법조작간편，준학도고、은정성화중복성량호，기괄용우미산해단저양품적함량측정，야괄용우산해후단저양품적함량측정，대수해단저류성분적함량측정구유보편괄용성。
Objective To establish a method for the determination of the hydrolyzed tannins from Juglans regia L ..Methods Gallic acid as a reference compound .UVspectrophotometry was used for the determination of the hydrolyzed tannins from Juglans regia L ..The modified Folin‐Ciocalteu method was set up ,then it was used to determine the contents of both the un‐hydrolyzed tannins and the hydrolyzed tannins .Results The standard curve equation was :Y=151 .8X+0 .041 ,and gallic acid was linear in the range of 0 .992‐4 .960 μg · mL -1 ,r=0 .999(n=6) .The optimal conditions for determining the hydrolyzed tannins from Juglans regia L .were as followed :1 .0 mL of the sample ,1 .5 mL of F‐C reagent ,10 mL of water ,with 70 g · L -1 sodium carbonate solution to the volume ,treatment at 30 ℃ for 1 .5 h .Conclusion This method is simple ,accurate ,stable and repeatable .It has universal applicability for the determination of both the un‐hydrolyzed tannins and the hydrolyzed tannins .