CAJ | 학술논문

目的：探讨细胞因子诱导的杀伤( cytokine-induced killer,CIK)细胞、树突状细胞( dendritic cells,DC)免疫治疗联合化疗对晚期卵巢癌患者外周血淋巴细胞亚群的影响,并评估其治疗效果。方法选取2011-09~2013-08就诊于新乡市中心医院的62例卵巢上皮性恶性肿瘤患者,其中30例患者采用DC-CIK联合化疗[紫杉醇( taxel)+卡铂( cisplatin)]为联合治疗组；选取同期进行单纯化疗的32例患者为单纯化疗组。3周为1个周期,3周期后评价疗效。治疗结束1周后采用流式细胞仪检测外周血CD3+、CD3+CD4+、CD4+/CD8+、CD4+CD25+T调节性T细胞、NK细胞的变化。结果每位患者治疗3个周期后,联合治疗组患者外周血CD3+、CD3+CD4+、CD4+/CD8+、NK水平明显高于单纯化疗组(P<0.05)。联合治疗组患者的客观缓解率为85.7%,显著高于单纯化疗组的56.5%(P<0.05)。联合治疗组患者的不良反应(包括骨髓抑制、肝功能损害)明显轻于单纯化疗组(P<0.05),联合治疗组患者治疗后体力状况评分较单纯化疗组改善明显。结论 DC-CIK细胞免疫治疗联合化疗能提高卵巢癌患者的细胞免疫功能,且安全有效。
목적：탐토세포인자유도적살상( cytokine-induced killer,CIK)세포、수돌상세포( dendritic cells,DC)면역치료연합화료대만기란소암환자외주혈림파세포아군적영향,병평고기치료효과。방법선취2011-09~2013-08취진우신향시중심의원적62례란소상피성악성종류환자,기중30례환자채용DC-CIK연합화료[자삼순( taxel)+잡박( cisplatin)]위연합치료조；선취동기진행단순화료적32례환자위단순화료조。3주위1개주기,3주기후평개료효。치료결속1주후채용류식세포의검측외주혈CD3+、CD3+CD4+、CD4+/CD8+、CD4+CD25+T조절성T세포、NK세포적변화。결과매위환자치료3개주기후,연합치료조환자외주혈CD3+、CD3+CD4+、CD4+/CD8+、NK수평명현고우단순화료조(P<0.05)。연합치료조환자적객관완해솔위85.7%,현저고우단순화료조적56.5%(P<0.05)。연합치료조환자적불량반응(포괄골수억제、간공능손해)명현경우단순화료조(P<0.05),연합치료조환자치료후체력상황평분교단순화료조개선명현。결론 DC-CIK세포면역치료연합화료능제고란소암환자적세포면역공능,차안전유효。
Objective To evaluate the curative effecacy of dentritic cell(DC)-cytokine induced killer cells(CIKs) combined with chem-otherapy in treatment of ovarian carcinoma by investigating the changes of T lymphocyte subsets in peripheral blood. Methods Clini-cal data and the changes of lymphocyte subsets in 62 patients with ovarian carcinoma,who were admitted to Central Hospital of Xinxiang from September 2011 to August 2013,were retrospectively reviewed. Thirty patients were treated by DC-CIK combined with chemothera-py( taxel+cisplatin) in combination group,while 32 patients were treated with chemotherapy alone in control group. Three weeks were designed as one course of treatment, and the curative effects were evaluated at 7 d after treatment. The immune functions ( CD3 +, CD3 +CD4 +,CD4 + /CD8 +,CD4 +CD25 +T,NK cells) were evaluated in two groups one week after treatment. Results After three courses of treatment,the levels of CD3 +,CD3 +CD4 +,CD4 + /CD8 +,NK cells in peripheral blood were statistically significantly higher in combination group than in control group(P<0. 05). The disease control rate(DCR)in combination group was higher than that in con-trol group(85. 7% vs 56. 5%,P<0. 05). The incidence of side effects(including bone marrow suppression,impaired liver function)was lower in combination group than in control group(P<0. 05). The physical condition and appetite of the patients in combination group were better than those in control group. Conclusion Treatment with DC-CIK cells immune therapy combined with chemotherapy can improve the immunological function in patinents with ovarian carcinoma,and it is safe and effective.