CAJ | 학술논문

本试验旨在研究饲料中镁添加量对中华绒螯蟹幼蟹生长、体成分及抗氧化能力的影响。在基础饲料中分别添加0、1.5、3.0、4.5、6.0和7.5 g/kg镁( L-天门冬氨酸镁形式)，制成镁含量实测值分别为0.98、1.43、3.40、4.79、6.65和7.98 g/kg的6种试验饲料。选取平均体重为(2.97±0.01) g的中华绒螯蟹幼蟹900只，随机分为6个组，每组5个重复，每个重复30只幼蟹。每组幼蟹随机投喂1种试验饲料，试验期为10周。结果表明：添加3.0 g/kg镁组幼蟹的增重率、存活率和全蟹粗蛋白质含量最高，但各组间差异不显著( P>0.05)。幼蟹日摄食率、饲料系数及全蟹粗脂肪、水分和粗灰分含量各组之间亦无显著差异( P>0.05)。全蟹中镁含量随着饲料中镁添加量的升高而升高，而钙含量则在未添加镁组最高，导致此组的钙镁比最高。幼蟹肝胰腺和血清中还原型谷胱甘肽( GSH )含量随着饲料中镁添加量的升高先升高后降低，在添加3.0 g/kg镁组达到最高值，其肝胰腺中GSH含量显著高于添加7.5 g/kg镁组( P<0.05)，血清中GSH含量显著高于未添加镁组( P<0.05)；血清和肝胰腺中丙二醛( MDA)含量则随着饲料中镁添加量的升高先降低后升高，在添加3.0 g/kg镁组获得最低值，显著低于其他各组( P<0.05)。由此得出，以L-天门冬氨酸镁为镁源，当饲料中镁添加量为3.0~4.5 g/kg时中华绒螯蟹幼蟹的生长效果较好；以肝胰腺中GSH含量为判据，采用二次回归模型进行拟合发现，当饲料中镁添加量为3.76 g/kg(饲料中镁含量为4.39 g/kg)时，中华绒螯蟹幼蟹的抗氧化能力最佳。
본시험지재연구사료중미첨가량대중화융오해유해생장、체성분급항양화능력적영향。재기출사료중분별첨가0、1.5、3.0、4.5、6.0화7.5 g/kg미( L-천문동안산미형식)，제성미함량실측치분별위0.98、1.43、3.40、4.79、6.65화7.98 g/kg적6충시험사료。선취평균체중위(2.97±0.01) g적중화융오해유해900지，수궤분위6개조，매조5개중복，매개중복30지유해。매조유해수궤투위1충시험사료，시험기위10주。결과표명：첨가3.0 g/kg미조유해적증중솔、존활솔화전해조단백질함량최고，단각조간차이불현저( P>0.05)。유해일섭식솔、사료계수급전해조지방、수분화조회분함량각조지간역무현저차이( P>0.05)。전해중미함량수착사료중미첨가량적승고이승고，이개함량칙재미첨가미조최고，도치차조적개미비최고。유해간이선화혈청중환원형곡광감태( GSH )함량수착사료중미첨가량적승고선승고후강저，재첨가3.0 g/kg미조체도최고치，기간이선중GSH함량현저고우첨가7.5 g/kg미조( P<0.05)，혈청중GSH함량현저고우미첨가미조( P<0.05)；혈청화간이선중병이철( MDA)함량칙수착사료중미첨가량적승고선강저후승고，재첨가3.0 g/kg미조획득최저치，현저저우기타각조( P<0.05)。유차득출，이L-천문동안산미위미원，당사료중미첨가량위3.0~4.5 g/kg시중화융오해유해적생장효과교호；이간이선중GSH함량위판거，채용이차회귀모형진행의합발현，당사료중미첨가량위3.76 g/kg(사료중미함량위4.39 g/kg)시，중화융오해유해적항양화능력최가。
This experiment was conducted to study the effects of magnesium ( Mg ) supplementation on growth, body composition and antioxidant ability of juvenile Chinese mitten crab ( Eriocheir sinensis) . Six ex-perimental diets were formulated with basal diet added of Mg ( the form of L-aspartic acid magnesium) of 0, 1.5, 3.0, 4.5, 6. 0 and 7. 5 g/kg, and the measured values of dietary Mg content were 0. 98, 1. 43, 3. 40, 4.79, 6.65 and 7. 98 g/kg, respectively. A total of 900 juvenile Chinese mitten crab with the average body weight of (2.97±0.01) g were randomly allocated to 6 groups with 5 replicates per group and 30 crab per rep-licate, and each group was randomly fed one of six experimental diets for 10 weeks. The results showed as fol-lows: the highest values of weight gain rate, survival ratio and crude protein content in whole-body were all found in the group with the Mg supplementation of 3.0 g/kg, but no significant differences in those indices a-mong all groups ( P>0.05) . There were also no significant differences in the daily feeding rate, feed conver-sion ratio, and the contents of moisture, crude lipid and ash in whole-body among all groups ( P>0.05) . The Mg content in whole-body was increased with the Mg supplementation increasing, while the highest value of calcium ( Ca) content in whole-body was appeared in the group without Mg supplementation, which lead to this group had the highest value of Ca/Mg in whole-body. The content of reduced glutathione ( GSH) in hepa-topancreas and serum was firstly increased and then decreased with the increase of dietary Mg supplementation, and the highest values were all found in the group with the Mg supplementation of 3.0 g/kg. The GSH content in hepatopancreas and serum in the group with the Mg supplementation of 3. 0 g/kg was significantly higher than that in the group with the Mg supplementation of 7.5 g/kg and the group without Mg supplementation, re-spectively (P<0.05). The content of malonaldehyde (MDA) in hepatopancreas and serum was firstly de-creased and then increased with the increase of dietary Mg supplementation, and the lowest values were found in the group with the Mg supplementation of 3. 0 g/kg, which was significantly lower than that in the other groups ( P<0.05) . In conclusion, the L-aspartic acid magnesium as Mg source, the juvenile Chinese mitten crab have the better growth effect when Mg supplementation is 3.0 g/kg;the GSH content in hepatopancreas as criterion, base on the quadratic regression model fitting, the juvenile Chinese mitten crab have the best antioxi-dant ability when Mg supplementation is 3.76 g/kg ( dietary Mg content is 4.39 g/kg) .