CAJ | 학술논문

以冬枣为试材，从发病的冬枣上提取出病原菌，并进行分离纯化和鉴定，明确导致冬枣发病的病原菌主要为酵母菌和根霉菌。使用滤纸片法测定5种植物精油（罗勒精油、松红梅精油、薰衣草精油、红百里香精油、迷迭香精油）对酵母菌和根霉菌的抑菌活性，结果表明：红百里香精油对酵母菌和根霉菌都具有很强的抑制作用，抑菌圈直径为（22．43±0．06）mm和（23．48±0．06）mm。另外，采用试管浓度稀释法对5种植物精油的最低抑菌浓度（M IC ）和最低杀菌浓度（M BC ）进行了测定，得知红百里香精油对酵母菌的M IC值是0．625μL／m L ，M BC值是1．25μL／m L ；对根霉菌的M IC是0．625μL／m L ， MBC值是2．5μL／mL。
이동조위시재，종발병적동조상제취출병원균，병진행분리순화화감정，명학도치동조발병적병원균주요위효모균화근매균。사용려지편법측정5충식물정유（라륵정유、송홍매정유、훈의초정유、홍백리향정유、미질향정유）대효모균화근매균적억균활성，결과표명：홍백리향정유대효모균화근매균도구유흔강적억제작용，억균권직경위（22．43±0．06）mm화（23．48±0．06）mm。령외，채용시관농도희석법대5충식물정유적최저억균농도（M IC ）화최저살균농도（M BC ）진행료측정，득지홍백리향정유대효모균적M IC치시0．625μL／m L ，M BC치시1．25μL／m L ；대근매균적M IC시0．625μL／m L ， MBC치시2．5μL／mL。
Essential oils have effective inhibitory activity with natural ,green and safe characteristics and good prospects of developing antiseptic and freshening agent for fruits and vegetables .Firstly ,the pathogens isolated and purified and then identified that yeasts and rhizopus were the main pathogens .Secondly ,antibacterial activities of five essential oils (basil oils ,leptospermum scoparium oil ,lavender essential oil ,thymus serpyllum oil ,rosemary oil)for yeasts and rhizopus were determined by filter papers .The result showed that thymus serpyllum oil had effec‐tive inhibition for yeast and rhizopus .The determines of inhibitory zones of them were 22 .43 mm ± 0 .06 mm and 23 .48 mm ± 0 .06 mm .Finally ,the minimum inhibitory concentration (MIC) and minimum bactericidal concentra‐tion (MBC) of the five essential oils were determined by diluting tube concentration .The MIC of thymus serpyllum oil for yeast was 0 .625μL/mL ,and the MBC of thymus serpyllum oil for yeast was 1 .25μL /mL .The MIC of thy‐mus serpyllum oil for rhizopus was 0 .625 μL/mL and the MBC of thymus serpyllum oil for rhizopus was 2 .5 μL/mL .