CAJ | 학술논문

目的：采用长期低剂量皮下注射鱼藤酮的方法制备SD大鼠帕金森病模型。方法将36只SD大鼠随机分为实验组24只和对照组12只，实验组给予l．5 mg／（ kg? d）鱼藤酮皮下注射，对照组注射等体积葵花油，连续30 d，每周停药1 d。给药结束后进行行为学测试，并断头取脑，采用免疫组化SP法检测中脑黑质多巴胺能神经元数目。结果对照组行为学改变不明显，其黑质多巴胺能神经元数目较多；实验组出现明显的行为学改变，其黑质多巴胺能神经元数目减少，符合PD的诊断标准。结论颈背部皮下注射鱼藤酮可成功制备SD大鼠帕金森病模型。
목적：채용장기저제량피하주사어등동적방법제비SD대서파금삼병모형。방법장36지SD대서수궤분위실험조24지화대조조12지，실험조급여l．5 mg／（ kg? d）어등동피하주사，대조조주사등체적규화유，련속30 d，매주정약1 d。급약결속후진행행위학측시，병단두취뇌，채용면역조화SP법검측중뇌흑질다파알능신경원수목。결과대조조행위학개변불명현，기흑질다파알능신경원수목교다；실험조출현명현적행위학개변，기흑질다파알능신경원수목감소，부합PD적진단표준。결론경배부피하주사어등동가성공제비SD대서파금삼병모형。
Objective To established PD models by long term subcutaneous injection of rotenone .Methods Totally 36 SD rats were randomly divided into two groups:control group contained 12 rats and model building group contained 24 rats.Rats in model building group were treated with rotenone at dose of 1.5 mg/(kg? d) by subcutaneous injection for 30 days, withdrawal one day a week .Rats in control group were treated with the same volume of sunflower oil .After the end of subcutaneous injection , the behavior changes of SD rats were tested and then sacrificing the rats to take out the brain tis -sues, detecting the expression of TH-positive cells in substantia nigra by counting out the number of TH-positive cells.Re-sults The behavior changes of SD rats in control group were not obvious , but the number of dopaminergic neurons in-creased.The behavior changes of SD rats in model building group was obvious , and their dopaminergic neurons numbers decreased .SD rats in model building group met the diagnostic criteria of PD .Conclusions The methods of long-term sub-cutaneous injection of rotenone could establish PD models in SD rats .