CAJ | 학술논문

本实验以花生籽仁为材料,对提取、纯化和利用高效液相色谱法分析测定花生籽仁中内源激素生长素(IAA)、脱落酸(ABA)、赤霉素(GA)和细胞分裂素(6-BA)含量的方法进行改进,针对花生籽仁中脂肪、酚类含量高等特点,对以往的 HPLC 分析植物内源激素的植物材料前处理方法进行了改进。试材用液氮研磨后以80%预冷的甲醇浸提,提取液利用旋转蒸发仪浓缩,氯仿去除色素,乙酸乙酯提取激素,交联聚乙烯吡咯烷酮(PVPP)去除酚类杂质,以甲醇和水为流动相,254nm吸收波长下检测内源激素含量。
본실험이화생자인위재료,대제취、순화화이용고효액상색보법분석측정화생자인중내원격소생장소(IAA)、탈락산(ABA)、적매소(GA)화세포분렬소(6-BA)함량적방법진행개진,침대화생자인중지방、분류함량고등특점,대이왕적 HPLC 분석식물내원격소적식물재료전처리방법진행료개진。시재용액담연마후이80%예랭적갑순침제,제취액이용선전증발의농축,록방거제색소,을산을지제취격소,교련취을희필각완동(PVPP)거제분류잡질,이갑순화수위류동상,254nm흡수파장하검측내원격소함량。
This experiment improved the method of extraction, purification and analysis with high-performance liquid chromatography ( HPLC) on endogenous hormones auxin (IAA), abscisic acid (ABA), gibberellic acid (GA) and 6-Benzylaminopurine (6-BA) content in peanut kernel.To deal with the existence of large amount of fat and phenolic, the test materials were grinded in liquid nitrogen and lixiviated in 80% methanol, then pigment was eliminated in chloroform, phytohormones were extracted in ethyl acetate, phenolic was compounded in polyving pyrrolidone (PVPP).Detection was made with UV detector at 254 nm.