CAJ | 학술논문

玫瑰（Rosa rugosa）组织色素较多，富含酚类物质。为了提取适于SRAP分析的高质量的玫瑰DNA，本实验基于CTAB法，在浓度2%和3%CTAB基础上，设计不同浓度的β-巯基乙醇和PVP抗氧化剂进行5种组合，研究了β-巯基乙醇和PVP对玫瑰DNA提取质量的影响。结果表明，在3%CTAB提取液中添加适量的β-巯基乙醇和PVP能获得纯度较高的玫瑰DNA，在组合III（3%CTAB、2%β-巯基乙醇+10%PVP）中，DNA电泳谱带清晰，无明显拖尾现象，SRAP分子标记效果较好，DNA的质量和纯度均满足于SRAP技术和DNA指纹图谱研究的要求。
매괴（Rosa rugosa）조직색소교다，부함분류물질。위료제취괄우SRAP분석적고질량적매괴DNA，본실험기우CTAB법，재농도2%화3%CTAB기출상，설계불동농도적β-구기을순화PVP항양화제진행5충조합，연구료β-구기을순화PVP대매괴DNA제취질량적영향。결과표명，재3%CTAB제취액중첨가괄량적β-구기을순화PVP능획득순도교고적매괴DNA，재조합III（3%CTAB、2%β-구기을순+10%PVP）중，DNA전영보대청석，무명현타미현상，SRAP분자표기효과교호，DNA적질량화순도균만족우SRAP기술화DNA지문도보연구적요구。
Rosa rugosa is the plant with high phenolic substance and pigment. In order to extract the high quality DNA suitable for SRAP analysis, the modified method was used with 5 kinds of combinations of different concentration of β-mer?captoethanol and PVP and 2%or 3%CTAB. The results showed that it can get the high purity rose DNA from the extracts of the combination of 3% CTAB+2% β-mercaptoethanol+10% PVP. Furthermore, the electrophoresis bands of DNA were clear and exhibited no significant tailing phenomenon. The average yield of rose DNA can meet the quality and purity in SRAP technical and DNA fingerprint requirements.