北京石油化工学院学报
北京石油化工學院學報
북경석유화공학원학보
JOURNAL OF BEIJING INSTITUTE OF PETRO-CHEMICAL TECHNOLOGY
2014年
4期
1-3
,共3页
高效液相色谱%白藜芦醇%葡萄酒
高效液相色譜%白藜蘆醇%葡萄酒
고효액상색보%백려호순%포도주
HPLC%resveratrol%wine
建立了高效液相色谱测定葡萄酒中白藜芦醇的方法。在C18为色谱柱,乙醇为溶剂,甲醇为流动相,流速为1 mL/min ,检测波长为306 nm的条件下,白藜芦醇的线性范围为0.2344~45μg/mL ,检测限为0.075μg/m L ,定量限为0.23μg/m L ,具有较好的精密度、稳定性和回收率。实验结果表明,该方法操作简便,分析快速,结果准确,可用于葡萄酒中白藜芦醇的定性、定量检测。
建立瞭高效液相色譜測定葡萄酒中白藜蘆醇的方法。在C18為色譜柱,乙醇為溶劑,甲醇為流動相,流速為1 mL/min ,檢測波長為306 nm的條件下,白藜蘆醇的線性範圍為0.2344~45μg/mL ,檢測限為0.075μg/m L ,定量限為0.23μg/m L ,具有較好的精密度、穩定性和迴收率。實驗結果錶明,該方法操作簡便,分析快速,結果準確,可用于葡萄酒中白藜蘆醇的定性、定量檢測。
건립료고효액상색보측정포도주중백려호순적방법。재C18위색보주,을순위용제,갑순위류동상,류속위1 mL/min ,검측파장위306 nm적조건하,백려호순적선성범위위0.2344~45μg/mL ,검측한위0.075μg/m L ,정량한위0.23μg/m L ,구유교호적정밀도、은정성화회수솔。실험결과표명,해방법조작간편,분석쾌속,결과준학,가용우포도주중백려호순적정성、정량검측。
A method for determination of resveratrol in wine by HPLC was proposed .The alcohol was solvent .The mobile phase was aqueous solution and 1 mL/min of speed .The detection was in 306 nm .The resveratrol linearity was found in range of 0.2344~45 μg/mL ,and the limit of detection and quantitation were 0.075 μg/mL and 0.23 μg/mL .It has good precision ,stability and percent recovery .Conclusion :The method is simple ,rapid and reproducible ,and could be used for determination of resveratrol in wine .