CAJ | 학술논문

从土壤中分离得到14种β葡萄糖苷酶高产菌株，其中菌株 YS2能够有效地将人参茎叶总皂苷和人参提取物中的人参皂苷转化为稀有人参皂苷 Rg3；菌株 YS2和 YS7的人参发酵物对结肠癌 colon26-M3．1细胞具有较强的抑制作用，其IC50值分别为180μg／mL和170μg／mL．根据ITS序列构建的系统发育树，确定菌株YS2为链格孢属（Alternaria）．
종토양중분리득도14충β포도당감매고산균주，기중균주 YS2능구유효지장인삼경협총조감화인삼제취물중적인삼조감전화위희유인삼조감 Rg3；균주 YS2화 YS7적인삼발효물대결장암 colon26-M3．1세포구유교강적억제작용，기IC50치분별위180μg／mL화170μg／mL．근거ITS서렬구건적계통발육수，학정균주YS2위련격포속（Alternaria）．
A total of 14 isolates of high-yieldβ-glycosidase microorganisms were isolated from soil.Among these isolates,strain YS2 shows a strong ability to convert total saponins of ginseng stems and leaves and ginseng extracts to minor ginsenoside Rg3.Fermented ginseng products by strain YS2 and YS7 could strongly inhibit the growth of colon 26-M3.1 cells and the IC50 values are 180μg/mL and 170μg/mL,respectively. Phylogenetic tree analysis based on ITS sequences show that the strain YS2 belongs to the genus Alternaria.