CAJ | 학술논문

目的：探讨肿瘤干细胞标志物乙醛脱氢酶（ALDH）在小鼠胰腺β细胞增殖模型中的表达情况。方法通过胰腺部分切除术构建小鼠胰腺β细胞增殖的模型，采用流式细胞术和免疫荧光检测其胰腺β细胞中ALDH的表达，采用Western blot检测细胞周期相关蛋白CyclinD1、CDK2及CDK4的表达。结果胰腺部分切除术后2周的胰岛细胞中aldefluor+细胞明显增多，Ki-67＋β细胞比例明显升高，且aldefluor+细胞CyclinD1、CyclinD2和CDK4蛋白表达水平均明显高于aldefluor－细胞。胰腺部分切除术后小鼠胰腺中的ALDH+细胞绝大部分均为β细胞。结论胰腺部分切除术诱导的增殖胰岛β细胞具有较高的ALDH活性，aldefluor荧光标记可能作为筛选增殖β细胞的标记物。
목적：탐토종류간세포표지물을철탈경매（ALDH）재소서이선β세포증식모형중적표체정황。방법통과이선부분절제술구건소서이선β세포증식적모형，채용류식세포술화면역형광검측기이선β세포중ALDH적표체，채용Western blot검측세포주기상관단백CyclinD1、CDK2급CDK4적표체。결과이선부분절제술후2주적이도세포중aldefluor+세포명현증다，Ki-67＋β세포비례명현승고，차aldefluor+세포CyclinD1、CyclinD2화CDK4단백표체수평균명현고우aldefluor－세포。이선부분절제술후소서이선중적ALDH+세포절대부분균위β세포。결론이선부분절제술유도적증식이도β세포구유교고적ALDH활성，aldefluor형광표기가능작위사선증식β세포적표기물。
Objective To investigate the expression of aldehyde dehydrogenase (ALDH) in the beta-cell proliferation model. Methods The presence of aldefluor+beta cells was detected by flow cytometry (FCM) and immunofluorescence in partial pancrea-tectomy (PPX) model. The expression of cell-cycle activators CDK2, CyclinD1 and CDK4 were detected by Western blot. Results The aldefluor+beta cells are essentially all positive for Ki-67 in PPX model, and expressed high levels of cell-cycle activators Cy-clinD1, CyclinD2 and CDK4. Conclusion Our data thus revealed that the proliferating beta cells induced by partial pancreatectomy showed a high ALDH activity. Moreover, our data also suggest that aldefluor lineage-tracing might be a marker for screening prolif-erating beta cells.