CAJ | 학술논문

目的：观察两种细胞辅助脂肪移植技术（Cell-assisted lipotransfer，CAL）隆乳的临床疗效。方法 CAL技术隆胸30例，根据术中提取的基质血管成分细胞（Stromal vascular fraction，SVF）来源分为两组，来源于脂肪组织的称为固相组（S组，n=24），来源于吸脂液的为液相组（L组，n=6）。术后均随访12个月。流式细胞仪检测两种来源SVF中脂肪干细胞（Adipose-derived stem cells，ADSCs）比例，MRI检测术前及术后6、12个月的乳房体积，计算移植脂肪组织的吸收率。结果两组新鲜分离的SVF细胞群中，ADSCs平均比例分别为S组40.73%、L组3.75%。 MRI测量及统计结果显示，S组术后6、12个月时乳房体积均明显大于术前（P<0.05），术后6个月与12个月时无明显差异（P>0.05）。12个月时脂肪吸收率为（51.83±15.28）%，与6个月时相比无明显差异（P>0.05）；L组12个月时脂肪吸收率为（75.47±12.20）%，与6个月时相比无明显差异（P>0.05）；MRI影像见囊肿2例，均来自S组。结论脂肪组织相比吸脂液，其SVF细胞群中ADSCs含量明显更高；CAL辅助脂肪移植技术隆乳术安全有效，术后6个月乳房体积即可保持基本稳定。
목적：관찰량충세포보조지방이식기술（Cell-assisted lipotransfer，CAL）륭유적림상료효。방법 CAL기술륭흉30례，근거술중제취적기질혈관성분세포（Stromal vascular fraction，SVF）래원분위량조，래원우지방조직적칭위고상조（S조，n=24），래원우흡지액적위액상조（L조，n=6）。술후균수방12개월。류식세포의검측량충래원SVF중지방간세포（Adipose-derived stem cells，ADSCs）비례，MRI검측술전급술후6、12개월적유방체적，계산이식지방조직적흡수솔。결과량조신선분리적SVF세포군중，ADSCs평균비례분별위S조40.73%、L조3.75%。 MRI측량급통계결과현시，S조술후6、12개월시유방체적균명현대우술전（P<0.05），술후6개월여12개월시무명현차이（P>0.05）。12개월시지방흡수솔위（51.83±15.28）%，여6개월시상비무명현차이（P>0.05）；L조12개월시지방흡수솔위（75.47±12.20）%，여6개월시상비무명현차이（P>0.05）；MRI영상견낭종2례，균래자S조。결론지방조직상비흡지액，기SVF세포군중ADSCs함량명현경고；CAL보조지방이식기술륭유술안전유효，술후6개월유방체적즉가보지기본은정。
Objective To evaluate the effect of two kinds of cell-assisted lipotransfer for breast augmentation. Methods Thirty patients accepted breast augmentation using cell-assisted lipotransfer (CAL), and completed 12-month follow-up. According to the stromal vascular fraction (SVF) distilled from adipose tissue or aspirated liquid, the patients were divided into 2 groups:S group and L group. Flow-cytometry was used to detect the percentage of adipose-derived stem cells (ADSCs) in distilled SVF. The breast volumes and images were evaluated by MRI pre-operative, 6 and 12 months post-operative. The data of breast volume were marked and the resorption rate of transplanted adipose tissue was calculated. Results Averagely, the percentage of ADSCs in freshly distilled SVF in S group is 40.73% and 3.75% in L group. MRI showed that the breast volume 6-month post-operative and 12-month post-operative were both larger than that of preoperative in S group (P<0.05), while no significant difference between the breast volume 6-month post-operative and 12-month post-operative were observed (P>0.05), neither the resorption rate of 6 and 12-month post-operative (P>0.05), and the resorption rate of transplanted adipose tissue 12-month post-operative in S group was (51.83 ±15.28)%. However, there were no significant difference of breast volume among three time points in L group (P>0.05), neither the resorption rate of 6 and 12-month post-operative (P>0.05), and the resorption rate of transplanted adipose tissue 12-month post-operative in L group was (75.47± 12.20)%. In addition, no calcification was seen in all MRI images but cysts were found in two cases of S group. Conclusion In process of breast augmentaion using cell-assissted lipotransfer, the distilled SVF from adipose tissue contains more ADSCs than that from aspirated liquid. The cell-assisted lipotransfer for breast augmentation is effective, safe, and the shrinkage stalls at 6-month post-operative.