CAJ | 학술논문

目的：观察洛铂对人宫颈癌细胞（Hela细胞）放射敏感性的影响，并初步探讨其机制。方法将体外培养的Hela细胞分为空白组、低浓度洛铂组、高浓度洛铂组、空白+照射组、低浓度洛铂+照射组、高浓度洛铂+照射组，单次照射6 Gy，比较各组细胞的存活情况，并通过流式细胞术检测和比较各组细胞的凋亡情况及细胞周期分布。结果洛铂对Hela细胞生长有抑制作用。空白组、低浓度洛铂组、高浓度洛铂组细胞的D0值分别为1.76、1.42、0.86 Gy（F=4006.0，P=0.00），Dq值分别为1.99、1.37、0.92 Gy（F=3087.0，P=0.00），α值分别为0.085、0.254、0.262 Gy-1（F=614.6，P=0.00），β值分别为0.0652、0.181、0.234 Gy-2（F=1342.1，P=0.00）。空白组、低浓度洛铂组、高浓度洛铂组、空白+照射组、低浓度洛铂+照射组、高浓度洛铂+照射组的细胞凋亡率分别为(1.10±0.01)％、(1.01±.006)％、(2.32±0.03)％、(2.87±0.008)％、(2.96±0.02)％、(4.38±0.01)％（F=23.1，P=0.00）。洛铂可以阻滞细胞于G2/M期。结论洛铂对人宫颈癌细胞有放射增敏作用，其机制可能与促进细胞凋亡有关。
목적：관찰락박대인궁경암세포（Hela세포）방사민감성적영향，병초보탐토기궤제。방법장체외배양적Hela세포분위공백조、저농도락박조、고농도락박조、공백+조사조、저농도락박+조사조、고농도락박+조사조，단차조사6 Gy，비교각조세포적존활정황，병통과류식세포술검측화비교각조세포적조망정황급세포주기분포。결과락박대Hela세포생장유억제작용。공백조、저농도락박조、고농도락박조세포적D0치분별위1.76、1.42、0.86 Gy（F=4006.0，P=0.00），Dq치분별위1.99、1.37、0.92 Gy（F=3087.0，P=0.00），α치분별위0.085、0.254、0.262 Gy-1（F=614.6，P=0.00），β치분별위0.0652、0.181、0.234 Gy-2（F=1342.1，P=0.00）。공백조、저농도락박조、고농도락박조、공백+조사조、저농도락박+조사조、고농도락박+조사조적세포조망솔분별위(1.10±0.01)％、(1.01±.006)％、(2.32±0.03)％、(2.87±0.008)％、(2.96±0.02)％、(4.38±0.01)％（F=23.1，P=0.00）。락박가이조체세포우G2/M기。결론락박대인궁경암세포유방사증민작용，기궤제가능여촉진세포조망유관。
Objective To investigate the effects of Lobaplatin on the radiosensitivity of human cervical carcinoma and its mechanisms. Methods Hela cells cultured invitro were assigned to the blank group, low concentration Lobaplatin group, high concentration Lobaplatin group, blank+radiation group, low concentration Lobaplatin+radiation group and high con-centration Lobaplatin+radiation group, single light 6 Gy. Flow cytometry was applied to detect the cell apoptosis and cell cycle. Results Lobaplatin had inhibition on the growth of cervical cancer Hela cells. The Do numericals of the blank group, low concentration Lobaplatin group and high concentration Lobaplatin group were 1.76, 1.42 and 0.86 Gy (F=4006.0, P=0.00) respectively, the Dq numericals were 1.99, 1.37 and 0.92 Gy (F=3087.0, P=0.00) respectively, theαnumericals were 0.085, 0.254 and 0.262 Gy-1 (F=614.6, P=0.00) respectively, theβnumericals were 0.0652, 0.181 and 0.234 Gy-2 (F=1342.1, P=0.00) respectively. The cell apoptosis rates of the above six groups were respectively (1.10±0.01)%、(1.01 ±.006)%、(2.32±0.03)%、(2.87±0.008)%、(2.96±0.02)%、(4.38±0.01)%(F=23.1, P=0.00) . Lobaplatin could block cells in G2/M phase. Conclusion Lobaplatin could intensify the radiosensitivity of human cervical carcinoma cell, through enhancing the apoptosis.