CAJ | 학술논문

为研究海洋防污损涂料添加剂Irogarol－1051的降解产物M2的基因毒性，应用微阵列技术，选取Affy－metrix公司鼠基因组2302．0基因芯片检测30μmol／L M2暴露下的鼠肝癌细胞基因表达变化．实验结果显示，96 h的M2暴露导致了38个基因在全部4组可能的对照／暴露中均发生显著变化（ p＜0．0025），其中只有Accn5基因研究较为透彻，该基因表达的抑制可能影响上皮钠通道的功能．此外，分别有10和82个功能注释基因在至少一组对照／暴露组中上调和下调．M2诱导的基因主要和细胞核（细胞成分）相关．M2抑制的基因则主要影响生物过程中的G蛋白偶联信号通路功能和细胞成分中的细胞膜内整合功能．
위연구해양방오손도료첨가제Irogarol－1051적강해산물M2적기인독성，응용미진렬기술，선취Affy－metrix공사서기인조2302．0기인심편검측30μmol／L M2폭로하적서간암세포기인표체변화．실험결과현시，96 h적M2폭로도치료38개기인재전부4조가능적대조／폭로중균발생현저변화（ p＜0．0025），기중지유Accn5기인연구교위투철，해기인표체적억제가능영향상피납통도적공능．차외，분별유10화82개공능주석기인재지소일조대조／폭로조중상조화하조．M2유도적기인주요화세포핵（세포성분）상관．M2억제적기인칙주요영향생물과정중적G단백우련신호통로공능화세포성분중적세포막내정합공능．
To better understand the toxicity of an antifouling booster biocide Irgarol-1051 degradation product M2 3-4-tert-butylamino-6-methylthiol-s-triazin-2-ylamino pro-pionaldehyde this study utilized a DNA microarray technique to explore the genotoxicity of M2. The Affymetrix Inc. rat genome 230 2.0 GeneChip was employed to examine alterations in gene regulation in rat hepatoma cells exposed to 30 μmol/L of M2 for 96 h.The results showed that 38 genes were significantly p<0.002 5 altered by M2 at two-fold changes in all the four possible control/exposure comparisons. Accn5 was the only well described gene consistently being suppressed which likely altered the epithelial sodium channel ENaC .10 and 82 annotated genes were up-and down-regulated in at least one of the control/exposure comparisons respectively. The induced genes were mainly involved in the nucleus belonging to the cellular component. The largest categories of suppression concerned G-protein coupled receptor protein signaling pathways belonging to the biological process and integral to membranes belonging to the cellular component.