CAJ | 학술논문

目的探讨门静脉动脉化（PVA）对肝硬化大鼠肝再生的影响。方法取50只肝硬化模型大鼠，按随机数字表法随机分为PVA组（40只）和对照组（10只）。PVA组行PVA+门-腔静脉分流术，对照组未行任何处理。检测每组大鼠术后或入组后1周(T1)、2周(T2)、4周(T3)、8周(T4)4个时间点的肝脏湿重与体重比、增殖细胞核抗原（PCNA）阳性肝细胞百分率、DNA合成期（S期）肝细胞百分率。组内各时间点比较采用单因素方差分析，两两比较采用LSD-t检验，两组间比较采用t检验。结果 PVA组大鼠T1、T2、T3、T4时间点的肝脏湿重与体重比分别为（3.72±0.26）%、（3.81±0.27）%、（3.83±0.31）%、（3.78±0.31）%，对照组为（2.84±0.37）%，PVA组较对照组明显增大（t=6.11，6.64，6.49，6.17；P<0.05）。PVA组T1、T2、T3、T4时间点的PCNA阳性肝细胞百分率分别为（76±6）%、（69±8）%、（20±5）%、（15±4）%，对照组为（11±2）%， PVA组较对照组明显增大（t=34.48，22.87，5.69，2.93；P<0.05）。随着时间的延长，PVA组内各时间点的PCNA阳性肝细胞百分率逐渐下降，差异有统计学意义（F=316.20，P<0.05）。PVA组T1、T2、T3、T4时间点的S期肝细胞百分率分别为（27.0±1.2）%、（20.5±1.4）%、（16.2±1.3）%、（13.5±1.3）%，对照组为（11.6±1.9）%，PVA组较对照组明显增大（t=21.97，12.15，6.30，2.68；P<0.05）。随着时间的延长，PVA组内各时间点的S期肝细胞百分率逐渐下降，差异有统计学意义（F=208.00，P<0.05）。结论 PVA能有效促进肝硬化大鼠肝再生，该效应随着时间的延长而下降。
목적탐토문정맥동맥화（PVA）대간경화대서간재생적영향。방법취50지간경화모형대서，안수궤수자표법수궤분위PVA조（40지）화대조조（10지）。PVA조행PVA+문-강정맥분류술，대조조미행임하처리。검측매조대서술후혹입조후1주(T1)、2주(T2)、4주(T3)、8주(T4)4개시간점적간장습중여체중비、증식세포핵항원（PCNA）양성간세포백분솔、DNA합성기（S기）간세포백분솔。조내각시간점비교채용단인소방차분석，량량비교채용LSD-t검험，량조간비교채용t검험。결과 PVA조대서T1、T2、T3、T4시간점적간장습중여체중비분별위（3.72±0.26）%、（3.81±0.27）%、（3.83±0.31）%、（3.78±0.31）%，대조조위（2.84±0.37）%，PVA조교대조조명현증대（t=6.11，6.64，6.49，6.17；P<0.05）。PVA조T1、T2、T3、T4시간점적PCNA양성간세포백분솔분별위（76±6）%、（69±8）%、（20±5）%、（15±4）%，대조조위（11±2）%， PVA조교대조조명현증대（t=34.48，22.87，5.69，2.93；P<0.05）。수착시간적연장，PVA조내각시간점적PCNA양성간세포백분솔축점하강，차이유통계학의의（F=316.20，P<0.05）。PVA조T1、T2、T3、T4시간점적S기간세포백분솔분별위（27.0±1.2）%、（20.5±1.4）%、（16.2±1.3）%、（13.5±1.3）%，대조조위（11.6±1.9）%，PVA조교대조조명현증대（t=21.97，12.15，6.30，2.68；P<0.05）。수착시간적연장，PVA조내각시간점적S기간세포백분솔축점하강，차이유통계학의의（F=208.00，P<0.05）。결론 PVA능유효촉진간경화대서간재생，해효응수착시간적연장이하강。
ObjectiveTo investigate the impacts of portal vein arterializations (PVA) on the liver regeneration in rats with liver cirrhosis.MethodsFifty rats with liver cirrhosis model were randomly divided into PVA group (n=40) and control group (n=10) according to the random number table method. Rats in PVA group underwent PVA+portocaval shunt. Rats in control group received no treatments. The ratio of hepatic wet weight to body weight, the hepatocyte percentage of positive proliferating cell nuclear antigen (PCNA) and hepatocyte percentage in DNA synthesis phase (S phase) in each group were measured at the time of 1 week (T1), 2 weeks (T2), 4 weeks (T3) and 8 weeks (T4) after operation or enrolling in the group. Parameters of every time points inside the group were compared by one-way analysis of variance and pairwise comparison was conducted by LSD-t test. Comparison between groups was conducted byt test.Results The ratio of hepatic wet weight to body weight at T1,T2,T3,T4 in PVA group [(3.72±0.26)%, (3.81±0.27)%, (3.83±0.31)%, (3.78±0.31)%] were signiifcantly increased compared with that in control group [(2.84±0.37)%] (t=6.11,6.64,6.49,6.17;P<0.05). The hepatocyte percentage of positive PCNA at T1, T2, T3, T4 in PVA group [(76±6)%, (69±8)%, (20±5)%, (15±4)% ] were signiifcantly increased compared with that in control group [(11±2)%] (t=34.48, 22.87,5.69,2.93;P<0.05). The hepatocyte percentage of positive PCNA at different time points inside the PVA group decreased gradually as time extended, where signiifcant difference was observed (F=316.20,P<0.05). The hepatocyte percentage in S phase at T1, T2, T3, T4 in PVA group [(27.0±1.2)%, (20.5±1.4)%, (16.2±1.3)%, (13.5±1.3)%] were signiifcantly increased compared with that in control group [(11.6±1.9)%] (t=21.97, 12.15, 6.30, 2.68;P<0.05). The hepatocyte percentage in S phase at different time points inside the PVA group decreased gradually as time extended, where signiifcant difference was observed (F=208.00,P<0.05).ConclusionsPVA can effectively promote liver regeneration in rats with liver cirrhosis and the effect declines as time extends.