中华肝脏外科手术学电子杂志
中華肝髒外科手術學電子雜誌
중화간장외과수술학전자잡지
CHINESE JOURNAL OF HEPATIC SURGERY(ELECTRONIC EDITION)
2014年
6期
43-46
,共4页
吉斐%华赟鹏%简佩恩%付顺军%赵坤%赖佳明%李绍强
吉斐%華赟鵬%簡珮恩%付順軍%趙坤%賴佳明%李紹彊
길비%화빈붕%간패은%부순군%조곤%뢰가명%리소강
再灌注损伤%大鼠%肝硬化,实验性%缺血后处理%T淋巴细胞亚群%白细胞介素4%白细胞介素10
再灌註損傷%大鼠%肝硬化,實驗性%缺血後處理%T淋巴細胞亞群%白細胞介素4%白細胞介素10
재관주손상%대서%간경화,실험성%결혈후처리%T림파세포아군%백세포개소4%백세포개소10
Reperfusion injury%Rats%Liver cirrhosis,experimental%Ischemic postconditioning%T-lymphocyte subsets%Interleukin-4%Interleukin-10
目的探讨缺血后处理(IPO)对肝硬化大鼠肝脏缺血-再灌注损伤(IRI)的保护作用及其免疫机制。方法按随机数字表法将30只肝硬化模型SD大鼠随机分为IPO组、IRI组和单纯肝切除组(肝切组),各10只。IPO组先切除40%肝脏,阻断第一肝门20 min,然后反复缺血-再灌注3次,最后持续再灌注;IRI组,切除40%肝脏,阻断第一肝门20 min后持续再灌注;肝切组切除40%肝脏。分别于术后6、24 h抽取大鼠下腔静脉血,检测ALT、AST、分化群(CD)4+、CD8+、调节性T细胞(Treg)百分率、白细胞介素(IL)-4、IL-10水平。3组比较采用单因素方差分析,两两比较采用LSD-t检验。结果恢复灌注6 h后,IPO组的ALT、AST分别为(1623±378)、(1993±469)U/L, IRI组相应为(2690±549)、(3020±577)U/L,IPO组ALT、AST明显低于IRI组(LSD-t=-4.21,-3.72;P<0.05)。恢复灌注24 h后,IPO组的ALT、AST分别为(307±76)、(555±137)U/L,IRI组相应为(518±105)、(1050±355) U/L,IPO组ALT、AST亦明显低于IRI组(LSD-t=-4.06,-3.37;P<0.05)。恢复灌注6 h后,IPO组CD4+、CD8+、CD4+/ CD8+比值、Treg、IL-4、 IL-10分别为(57±5)%、(25±3)%、2.3±0.5、(8.9±0.4)%、(1.27±0.25)mg/L、(0.61±0.03)mg/L,IRI组相应为(52±6)%、(12±3)%、4.5±0.8、(7.3±0.3)%、(0.66±0.11)mg/L、(0.34±0.06)mg/L,IPO组CD8+、Treg、IL-4、IL-10较IRI组明显升高,CD4+/ CD8+比值明显降低(LSD-t=7.74,6.67,5.52,9.31,-6.69;P<0.05)。结论 IPO可能通过减轻免疫损伤发挥对肝硬化大鼠肝脏IRI的保护作用。
目的探討缺血後處理(IPO)對肝硬化大鼠肝髒缺血-再灌註損傷(IRI)的保護作用及其免疫機製。方法按隨機數字錶法將30隻肝硬化模型SD大鼠隨機分為IPO組、IRI組和單純肝切除組(肝切組),各10隻。IPO組先切除40%肝髒,阻斷第一肝門20 min,然後反複缺血-再灌註3次,最後持續再灌註;IRI組,切除40%肝髒,阻斷第一肝門20 min後持續再灌註;肝切組切除40%肝髒。分彆于術後6、24 h抽取大鼠下腔靜脈血,檢測ALT、AST、分化群(CD)4+、CD8+、調節性T細胞(Treg)百分率、白細胞介素(IL)-4、IL-10水平。3組比較採用單因素方差分析,兩兩比較採用LSD-t檢驗。結果恢複灌註6 h後,IPO組的ALT、AST分彆為(1623±378)、(1993±469)U/L, IRI組相應為(2690±549)、(3020±577)U/L,IPO組ALT、AST明顯低于IRI組(LSD-t=-4.21,-3.72;P<0.05)。恢複灌註24 h後,IPO組的ALT、AST分彆為(307±76)、(555±137)U/L,IRI組相應為(518±105)、(1050±355) U/L,IPO組ALT、AST亦明顯低于IRI組(LSD-t=-4.06,-3.37;P<0.05)。恢複灌註6 h後,IPO組CD4+、CD8+、CD4+/ CD8+比值、Treg、IL-4、 IL-10分彆為(57±5)%、(25±3)%、2.3±0.5、(8.9±0.4)%、(1.27±0.25)mg/L、(0.61±0.03)mg/L,IRI組相應為(52±6)%、(12±3)%、4.5±0.8、(7.3±0.3)%、(0.66±0.11)mg/L、(0.34±0.06)mg/L,IPO組CD8+、Treg、IL-4、IL-10較IRI組明顯升高,CD4+/ CD8+比值明顯降低(LSD-t=7.74,6.67,5.52,9.31,-6.69;P<0.05)。結論 IPO可能通過減輕免疫損傷髮揮對肝硬化大鼠肝髒IRI的保護作用。
목적탐토결혈후처리(IPO)대간경화대서간장결혈-재관주손상(IRI)적보호작용급기면역궤제。방법안수궤수자표법장30지간경화모형SD대서수궤분위IPO조、IRI조화단순간절제조(간절조),각10지。IPO조선절제40%간장,조단제일간문20 min,연후반복결혈-재관주3차,최후지속재관주;IRI조,절제40%간장,조단제일간문20 min후지속재관주;간절조절제40%간장。분별우술후6、24 h추취대서하강정맥혈,검측ALT、AST、분화군(CD)4+、CD8+、조절성T세포(Treg)백분솔、백세포개소(IL)-4、IL-10수평。3조비교채용단인소방차분석,량량비교채용LSD-t검험。결과회복관주6 h후,IPO조적ALT、AST분별위(1623±378)、(1993±469)U/L, IRI조상응위(2690±549)、(3020±577)U/L,IPO조ALT、AST명현저우IRI조(LSD-t=-4.21,-3.72;P<0.05)。회복관주24 h후,IPO조적ALT、AST분별위(307±76)、(555±137)U/L,IRI조상응위(518±105)、(1050±355) U/L,IPO조ALT、AST역명현저우IRI조(LSD-t=-4.06,-3.37;P<0.05)。회복관주6 h후,IPO조CD4+、CD8+、CD4+/ CD8+비치、Treg、IL-4、 IL-10분별위(57±5)%、(25±3)%、2.3±0.5、(8.9±0.4)%、(1.27±0.25)mg/L、(0.61±0.03)mg/L,IRI조상응위(52±6)%、(12±3)%、4.5±0.8、(7.3±0.3)%、(0.66±0.11)mg/L、(0.34±0.06)mg/L,IPO조CD8+、Treg、IL-4、IL-10교IRI조명현승고,CD4+/ CD8+비치명현강저(LSD-t=7.74,6.67,5.52,9.31,-6.69;P<0.05)。결론 IPO가능통과감경면역손상발휘대간경화대서간장IRI적보호작용。
ObjectiveTo investigate the protective effects and its mechanism of ischemia postconditioning (IPO) in hepatic ischemia-reperfusion injury (IRI) in liver cirrhotic rats.MethodsThirty liver cirrhotic Sprague-Dawley (SD) rats were randomly divided into three groups according to the random table methods: ischemia postconditioning (IPO) group, IRI group, and pure hepatectomy (PH) group with 10 rats in each group. Rats in IPO group underwent partial hepatectomy (40%), and the first portal was occluded for 20 min, then 3 times of ischemia-reperfusion were performed, and ifnally continuous reperfusion. Rats in IRI group underwent partial hepatectomy (40%), and the ifrst portal was occluded for 20 min, then continuous reperfusion was performed. Rats in PH group underwent partial hepatectomy (40%) only. Inferior vena cava blood was collected at 6, 24 h after operation. Levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), proportion of cluster of differentiation (CD) 4+, CD8+, regulatory T cells (Treg) and levels of interleukin (IL)-4, IL-10 were tested. Comparison of three groups was conducted by one way analysis of variance and pairwise comparison by LSD-t test.ResultsAfter 6 h reperfusion, levels of ALT and AST in IPO group [(1 623±378) , (1 993±469) U/L] were signiifcantly lower than those in IRI group [(2 690±549) , (3 020±577) U/L] (LSD-t=-4.21,-3.72; P<0.05). After 24 h reperfusion, levels of ALT and AST in IPO group [(307±76) , (555±137) U/L] were still signiifcantly lower than those in IRI group [(518±105) , (1 050±355) U/L] (LSD-t=-4.06,-3.37;P<0.05). After 6 h reperfusion, proportion of CD4+, CD8+, CD4+/CD8+ ratio, Treg, and levels of IL-4, IL-10 in IPO group were (57±5) %, (25±3) %, 2.3±0.5, (8.9±0.4) %, (1.27±0.25) mg/L, (0.61±0.03) mg/L, respectively. While in IRI group, they were (52±6)%, (12±3) %, 4.5±0.8, (7.3±0.3) %, (0.66±0.11) mg/L, (0.34±0.06) mg/L, respectively. In IPO group, CD8+, Treg, IL-4 and IL-10 increased signiifcantly, while CD4+/CD8+ ratio decreased signiifcantly, compared with those in IRI group (LSD-t= 7.74, 6.67, 5.52, 9.31,-6.69;P<0.05).ConclusionIPO can prevent from hepatic IRI injury in liver cirrhotic rats through decreasing the immune injury.