甘薯羽状斑驳病毒和褪绿矮化病毒双重 RT-PCR 检测方法的建立
감서우상반박병독화퇴록왜화병독쌍중 RT-PCR 검측방법적건립
Dfvflopmfnt of Dutiplfx RT-PCR for thf Dftfction of SPFMV and SPCSV in Swfft Potato
  • 万方数据
    福建农业学报 복건농업학보
    FUJIAN JOURNAL OF AGRICULTURAL SCIENCES
    2014年 11期 1114-1117 ,共4页

    许泳清%李华伟%邱思鑫%刘中华%邱永祥%罗文彬%纪荣昌%汤浩%余华

    허영청%리화위%구사흠%류중화%구영상%라문빈%기영창%탕호%여화

    甘薯%甘薯羽状斑驳病毒%甘薯褪绿矮化病毒%双重 RT-PCR 甘藷%甘藷羽狀斑駁病毒%甘藷褪綠矮化病毒%雙重 RT-PCR
    감서%감서우상반박병독%감서퇴록왜화병독%쌍중 RT-PCR
    sweet potato%SPCSV%SPFMV%duplex RT-PCR
    根据 GenBank 中公布的甘薯羽状斑驳病毒（Sweet potato feathery mottle virus，SPFMV）外壳蛋白（CP ）基因和甘薯褪绿矮化病毒（Sweet potato chlorotic stunt virus，SPCSV）热激蛋白（Hsp70）基因序列保守区域设计了2对特异性引物，通过 RT-PCR 反应程序的优化，建立了能同时检测 SPFMV 和 SPCSV 的双重 PCR检测体系。该检测体系能够1次扩增出 SPFMV 和 SPCSV 的特异性片段，片段大小为461 bp 和304 bp，测序结果表明，2种病毒序列与参考序列的同源性达93％以上。
    근거 GenBank 중공포적감서우상반박병독（Sweet potato feathery mottle virus，SPFMV）외각단백（CP ）기인화감서퇴록왜화병독（Sweet potato chlorotic stunt virus，SPCSV）열격단백（Hsp70）기인서렬보수구역설계료2대특이성인물，통과 RT-PCR 반응정서적우화，건립료능동시검측 SPFMV 화 SPCSV 적쌍중 PCR검측체계。해검측체계능구1차확증출 SPFMV 화 SPCSV 적특이성편단，편단대소위461 bp 화304 bp，측서결과표명，2충병독서렬여삼고서렬적동원성체93％이상。
    A duplex PCR protocol for the detection of two main viruses in sweet potato was developed based on the coat protein (CP )gene of Sweet potato feathery mottle virus (SPFMV)and the (Hsp70)gene of Sweet potato chlorotic stunt virus (SPCSV).They were able to amplify unique DNA fragments of 461 bp and 304bp in a single PCR reaction.The sequence analysis suggst that the two viruses shared at least 93 percent homology with other related viruses.
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