CAJ | 학술논문

以秋水仙素为诱变剂，结合组织培养技术，对文心兰丛生芽和原球茎进行诱变试验，并应用分子标记技术检测诱变后代的变异情况。结果表明：培养基添加秋水仙素混合培养法是文心兰丛生芽诱变的最佳途径，以添加500 mg·L－1的秋水仙素诱导10 d 效果最佳，植株变异率高达26．7％，变异植株类型包括植株粗壮、多叶、叶片变宽、变厚、矮化、叶片线艺等；经5～6次继代培养，部分变异株系仍保持稳定遗传性状，其中筛选出的稳定绿叶金边、金叶绿边变异株系 RAPD 标记表明其在 DNA 水平上发生了变异，为文心兰育种提供了新的方法和思路。
이추수선소위유변제，결합조직배양기술，대문심란총생아화원구경진행유변시험，병응용분자표기기술검측유변후대적변이정황。결과표명：배양기첨가추수선소혼합배양법시문심란총생아유변적최가도경，이첨가500 mg·L－1적추수선소유도10 d 효과최가，식주변이솔고체26．7％，변이식주류형포괄식주조장、다협、협편변관、변후、왜화、협편선예등；경5～6차계대배양，부분변이주계잉보지은정유전성상，기중사선출적은정록협금변、금협록변변이주계 RAPD 표기표명기재 DNA 수평상발생료변이，위문심란육충제공료신적방법화사로。
With colchicine was used as the mutagenic agent, combined with tissue culture techniques, the mutagenesis experiments for bud clump and PLB of Oncidium and the molecular marker technology to detect mutagenesis progeny were studied.Results showed that method of mixed medium supplemented with colchicine was the best way to mutage the Oncidium,and the best condition was 10d induction with 500 mg·L-1 of colchicine. Plant mutation rate was as high as 26.7%,and there were different mutation forms,such as hairchested, polyphyllous,dwarfed plantlets and widened,thickened leaves,and linear,patterned leaves.Mutant strain went through 5~6 successive transfer culture,and part of them remained properties well.Among them,the variation with golden margin leaves and variation with green margin golden leaves had apparent variation compared with the original species on DNA level by RAPD markers.It suggested a new breeding methods and ideas for Oncidium .