福建农业学报
福建農業學報
복건농업학보
FUJIAN JOURNAL OF AGRICULTURAL SCIENCES
2014年
11期
1058-1061
,共4页
程龙飞%傅光华%施少华%陈红梅%牟巍%杜金铃%王贵华%傅秋玲%万春和%黄瑜
程龍飛%傅光華%施少華%陳紅梅%牟巍%杜金鈴%王貴華%傅鞦玲%萬春和%黃瑜
정룡비%부광화%시소화%진홍매%모외%두금령%왕귀화%부추령%만춘화%황유
鸭疫里默氏菌%鸭传染性浆膜炎%灭活疫苗
鴨疫裏默氏菌%鴨傳染性漿膜炎%滅活疫苗
압역리묵씨균%압전염성장막염%멸활역묘
Riemerella anatipestifer%duck infectious serositis%the inactivated vaccine
为研制鸭传染性浆膜炎二价灭活疫苗，以鸭疫里默氏菌血清1型 RAf63株和2型 RAf34株为菌种，培养，灭活，浓缩，混合后制成二价灭活疫苗。2倍量接种6 d 樱桃谷鸭测定疫苗的安全性；接种6 d 樱桃谷鸭，免疫后5、10、14、21、35、49、63、70 d，进行攻菌试验，测定疫苗的免疫原性。结果表明，该疫苗安全，免疫后14 d 产生保护，保护率为90％，免疫持续期可达60 d。研制成功可用于预防由血清1型和2型鸭疫里默氏菌引起的鸭传染性浆膜炎。
為研製鴨傳染性漿膜炎二價滅活疫苗，以鴨疫裏默氏菌血清1型 RAf63株和2型 RAf34株為菌種，培養，滅活，濃縮，混閤後製成二價滅活疫苗。2倍量接種6 d 櫻桃穀鴨測定疫苗的安全性；接種6 d 櫻桃穀鴨，免疫後5、10、14、21、35、49、63、70 d，進行攻菌試驗，測定疫苗的免疫原性。結果錶明，該疫苗安全，免疫後14 d 產生保護，保護率為90％，免疫持續期可達60 d。研製成功可用于預防由血清1型和2型鴨疫裏默氏菌引起的鴨傳染性漿膜炎。
위연제압전염성장막염이개멸활역묘，이압역리묵씨균혈청1형 RAf63주화2형 RAf34주위균충，배양，멸활，농축，혼합후제성이개멸활역묘。2배량접충6 d 앵도곡압측정역묘적안전성；접충6 d 앵도곡압，면역후5、10、14、21、35、49、63、70 d，진행공균시험，측정역묘적면역원성。결과표명，해역묘안전，면역후14 d 산생보호，보호솔위90％，면역지속기가체60 d。연제성공가용우예방유혈청1형화2형압역리묵씨균인기적압전염성장막염。
The purpose of this study was to develop a bivalent inactivated vaccine against duck infectious serositis. Riemerella anatipestifer strain RAf63 (serotype 1)and RAf34 (serotype 2)were used as the vaccine strains. The strains were cultured and inactivated and concentrated respectively,and then mixed. The bivalent inactivated vaccine was made from the mixture. A double dose of inoculation to 6-day old ducklings were used to determine the safety of the vaccine. To determine the immunogenicity of the vaccine,6-day old ducklings were vaccinated with the vaccine and challenged with the vaccine strains on the 5 th ,10th ,14th ,21 th ,35 th ,49 th ,63 th ,and 70th d postinoculation, respectively. The results showed that the vaccine was safe and the protection rate was 90% on the 14th day postinoculation and the vaccine immunity duration was 60 days. The vaccine can be used to prevent duck infectious serositis caused by Riemerella anatipestifer serotype 1 and 2.
