CAJ | 학술논문

目的：探讨单核细胞趋化蛋白-1（MCP-1）在糖尿病肾病大鼠肾组织中的表达及厄贝沙坦联合普罗布考对其表达的影响。方法采用高糖高脂高胆固醇饮食并配合链脲佐菌素（STZ）腹腔注射的方法建立2型糖尿病肾病大鼠模型。将大鼠随机分为正常对照组（NC）、糖尿病肾病组（DN）、厄贝沙坦组（DI），厄贝沙坦+普罗布考组（DP），检测各组大鼠体重、随机血糖、24h尿量、24h尿蛋白定量（24hUTP）、SCR、BUN，行HE染色观察大鼠肾脏一般结构；免疫组化观察MCP-1的表达；RT-PCR观察MCP-1mRNA的表达。结果与NC组比较，各组大鼠肾脏病理损伤加重，24h尿量、24hUTP、随机血糖、SCR、BUN、肾脏MCP-1mRNA及蛋白均明显增加，体重减轻（P<0.05）；与DN组比较，DI组、DP组大鼠肾脏病理损伤减轻，24h尿量、24hUTP、肾脏MCP-1mRNA及蛋白下降，体重增加（P<0.05）；与DI组比较，DP组大鼠肾脏病理损伤减轻，24h尿量、24hUTP、肾脏MCP-1mRNA及蛋白水平下降（P<0.05）；其余指标无明显改善（P>0.05）。结论 MCP-1参与了糖尿病肾病的发生发展；厄贝沙坦及普罗布考能够降低糖尿病肾病MCP-1的表达，缓解肾脏病理损伤，联合后效果更明显。
목적：탐토단핵세포추화단백-1（MCP-1）재당뇨병신병대서신조직중적표체급액패사탄연합보라포고대기표체적영향。방법채용고당고지고담고순음식병배합련뇨좌균소（STZ）복강주사적방법건립2형당뇨병신병대서모형。장대서수궤분위정상대조조（NC）、당뇨병신병조（DN）、액패사탄조（DI），액패사탄+보라포고조（DP），검측각조대서체중、수궤혈당、24h뇨량、24h뇨단백정량（24hUTP）、SCR、BUN，행HE염색관찰대서신장일반결구；면역조화관찰MCP-1적표체；RT-PCR관찰MCP-1mRNA적표체。결과여NC조비교，각조대서신장병리손상가중，24h뇨량、24hUTP、수궤혈당、SCR、BUN、신장MCP-1mRNA급단백균명현증가，체중감경（P<0.05）；여DN조비교，DI조、DP조대서신장병리손상감경，24h뇨량、24hUTP、신장MCP-1mRNA급단백하강，체중증가（P<0.05）；여DI조비교，DP조대서신장병리손상감경，24h뇨량、24hUTP、신장MCP-1mRNA급단백수평하강（P<0.05）；기여지표무명현개선（P>0.05）。결론 MCP-1삼여료당뇨병신병적발생발전；액패사탄급보라포고능구강저당뇨병신병MCP-1적표체，완해신장병리손상，연합후효과경명현。
ObjectiveThis experiment studied the expression of monocyte chemoattractant protein-1(MCP-1)in Kidney damaged diabetic nephropathy rats and the effect of irbesartan and probucol.MethodsUsing the high-sugar,high-fat and high cholesterol diets combined with intraperitoneal injection of Streptozotocin(STZ)to establish diabetic nephropathy rats model. Rats were randomly divided into normal control(NC)group,diabetic nephropathy(DN)group,irbesartan(DI)group,irbesartan and Probucol(DP)group;Testing weight,blood glucose,24h urine volume,24h urine protein quantitative(24UTP), blood muscle anhydride(SCR)and urea nitrogen(BUN); Using HE stain to observe pathological morphology of each group;immunohistochemistry was applied to observe the protein expression of MCP-1;RT-PCR to observe MCP-1mRNA expression. ResultsCompared with NC group,all of groups the rats Kidney tissue pathology were exacerbated,the rats of 24h urine volume, 24UTP,BG,SCR,BUN,expression of MCP-1mRNA and protein in the renal tissues were significantly increased,loss of weight(P<0.05); Compared with DN group,DI group and DP group the rats Kidney tissue pathology were improved,24h urine volume,24UTP,expression of MCP-1 mRNA and protein in the renal tissues were decreased,put on weight(P<0.05);Compared with the DI group,DP group the rats Kidney tissue pathology were improved,24h urine volume,24UTP,expression of MCP-1 mRNA and protein in the renal tissues were decreased(P<0.05);The rest of the indicators has no significant Changed(P>0.05). ConclusionMCP-1 play an important role in the occurrence and development of diabetic nephropathy;Irbesartan and Probucol can reduce expressions of MCP-1 in diabetic renal tissues,to some extent relieving the pathological damage of the kidney,after joint of them are more obvious effect.