CAJ | 학술논문

为了检测烯脂酰辅酶A水合酶短链1（ECHS1）的甲基化状态，以同源比对克隆的方法获得荷斯坦奶牛ECHS1部分片段的序列，首先预测甲基化数目，然后采用重亚硫酸盐转化测序（BS-Seq）的方法检测了所获得片段的甲基化状态。结果表明，该试验成功获得了ECHS1459 bp的序列，其C+G含量为53.88％，含有28个CpG岛，其中1号、2号、9号、11号、13号、14号、17号、22号、24号、27号位点所有个体都发生了甲基化。
위료검측희지선보매A수합매단련1（ECHS1）적갑기화상태，이동원비대극륭적방법획득하사탄내우ECHS1부분편단적서렬，수선예측갑기화수목，연후채용중아류산염전화측서（BS-Seq）적방법검측료소획득편단적갑기화상태。결과표명，해시험성공획득료ECHS1459 bp적서렬，기C+G함량위53.88％，함유28개CpG도，기중1호、2호、9호、11호、13호、14호、17호、22호、24호、27호위점소유개체도발생료갑기화。
In order to detect the methylation status of enoyl-CoA hydratase short chain 1 (ECHS1), the sequence fragments of ECHS1 was obtained for Holstein cows by homologous cloning. The number of methylation was firstly predicted, and then methylation status of the obtained sequence fragments was detected by method of sequencing of bisulfite conversion. The results showed that fragments of ECHS1 with the size of 459 bp were obtained. C+G content was 53.88%. The fragments contained 28 CpGs islands and all individuals at sites 1, 2, 9, 11, 13, 14, 17, 22, 24 and 27 had been methylated.