CAJ | 학술논문

对不同细胞进行牛血清中牛源病毒检测的比较实验，选出用于牛血清中病毒检测的最适合外源病毒增殖的细胞，从而提高血清中病毒的检出率，以确保用于疫苗生产的牛血清安全可靠。将牛腹泻病毒、牛腺病毒、牛细小病毒、牛副流感病毒、呼肠孤病毒和狂犬病毒接种到培养好的MDBK细胞、VERO细胞、BT细胞中，逐日观察细胞的病变情况，待观察到10％细胞出现病变或者培养7 d后，进行免疫荧光染色，选出适合用于六种病毒增殖的细胞系。结果显示，BT细胞对所有病毒敏感，VERO细胞对BAV、BVDV病毒不敏感，MDBK对REO、BVDV、RV三种病毒不敏感。综合比较后，选择BT细胞为BPIV、BPV、BAV、BVDV的增殖及实验用细胞，VERO细胞为REO、RV的增殖及实验用细胞。
대불동세포진행우혈청중우원병독검측적비교실험，선출용우우혈청중병독검측적최괄합외원병독증식적세포，종이제고혈청중병독적검출솔，이학보용우역묘생산적우혈청안전가고。장우복사병독、우선병독、우세소병독、우부류감병독、호장고병독화광견병독접충도배양호적MDBK세포、VERO세포、BT세포중，축일관찰세포적병변정황，대관찰도10％세포출현병변혹자배양7 d후，진행면역형광염색，선출괄합용우륙충병독증식적세포계。결과현시，BT세포대소유병독민감，VERO세포대BAV、BVDV병독불민감，MDBK대REO、BVDV、RV삼충병독불민감。종합비교후，선택BT세포위BPIV、BPV、BAV、BVDV적증식급실험용세포，VERO세포위REO、RV적증식급실험용세포。
Choose the most suitable cell that was used in testing for exogenous virus in bovine serum, then as sensitive cells for detecting the virus in bovine serum in order to improve the detection rate of virus in the serum, it ensured that the bovine serum which was used in production of vaccine was safe. Be ready to cultivate appropriate cells including MDBK, VERO, BT. At the same time,six kinds of virus referred to the BPV, BVDV, BAV, BPIV, REO, RV, Six kinds of virus was inoculated on these three cells. Observing the cells which was inoculated virus every day. After 10% cell was appeared lesion or the cell was cultivated for 7 days, take out the cell, and these cells were dyeing by immunofluorescence, then according to the staining results to determine which cell was suitable for detecting these virus. The results showed that BT cell was sensitive to all cells, Vero cell was not sensitive to BAV, BVDV, MDBK was not sensitive to REO, BVDV, RV. The results of this study showed that BT cells can be used in proliferation and testing for BPV, BPIV, BAV, BVDV, and Vero cell can be used for REO, RV. In order to improve the detection rate of virus in the serum, we can choose a variety of cells at the same time.