CAJ | 학술논문

建立了17种兽药中非法添加氨茶碱和二羟丙茶碱的高效液相色谱法。以十八烷基键合硅胶为填充剂，0．025 mol／L磷酸二氢钾溶液－甲醇为流动相，二极管阵列检测器全波长（200～400 nm）扫描，270 nm进行测定，并采用峰纯度检查和光谱相似度检查辅助对照品比对方法，对非法添加药物进行确证。在此液相色谱条件下，氨茶碱和二羟丙茶碱与其他物质峰能很好的分离，两种药物在0．5～200μg／mL范围内线性关系良好，相关系数r均为1．0000。按外标法以峰面积计算，氨茶碱和二羟丙茶碱的平均回收率分别为92．6％～108．3％、93．5％～105．9％， RSD 分别为0．05％～3．6％、0．2％～3．7％。结果表明，该方法适合兽药中氨茶碱和二羟丙茶碱的检测。
건립료17충수약중비법첨가안다감화이간병다감적고효액상색보법。이십팔완기건합규효위전충제，0．025 mol／L린산이경갑용액－갑순위류동상，이겁관진렬검측기전파장（200～400 nm）소묘，270 nm진행측정，병채용봉순도검사화광보상사도검사보조대조품비대방법，대비법첨가약물진행학증。재차액상색보조건하，안다감화이간병다감여기타물질봉능흔호적분리，량충약물재0．5～200μg／mL범위내선성관계량호，상관계수r균위1．0000。안외표법이봉면적계산，안다감화이간병다감적평균회수솔분별위92．6％～108．3％、93．5％～105．9％， RSD 분별위0．05％～3．6％、0．2％～3．7％。결과표명，해방법괄합수약중안다감화이간병다감적검측。
A method for the determination of aminophylline and diprophylline in 17 kinds of veterinary drugs was developed by the high performance liquid chromatography with photo-diode array detector ( HPLC-PDA) . It was tested with C18 column, using 0.025 mol/L potassium dihydrogen phosphate solution and methanol as the mobile phase. The detection wavelength was set at 270 nm and simultaneously collected the spectrum at wavelength of 200~400 nm. Peak purity test and spectrum similar test were helped to identify the aminophylline and diprophylline. The concentration of the aminophylline and diprophylline in the range of 0.5~200μg/mL showed a good linear relationship, r=1.0000. The average recoveries of the drugs ranged from 92.6% to 108.3% at spiked levels with RSD less than 3. 7%. The results showed that the method was suitable for detection of aminophylline and diprophylline in veterinary drugs.