江西医药
江西醫藥
강서의약
JIANGXI MEDICAL JOURNAL
2014年
12期
1410-1413
,共4页
王志强%喻国平%周斌锋%彭兵锋
王誌彊%喻國平%週斌鋒%彭兵鋒
왕지강%유국평%주빈봉%팽병봉
食管癌%sFRP%WIF-1%CD133%CD44
食管癌%sFRP%WIF-1%CD133%CD44
식관암%sFRP%WIF-1%CD133%CD44
Esophageal Cancer%sFRP%WIF-1%CD133%CD44
目的：探讨sFRP、WIF-1、CD133、CD44在食管癌中的表达与临床病理特征之间的关系，为食管癌的研究提供新思路。方法选取2013年1月-2013年8月经手术切除的食管癌标本40例，术后病理均证实为食管癌患者，采用RT-PCR方法分析sFRP（分泌型卷曲相关蛋白）、WIF-1（Wnt抑制因子-1）、CD133（白细胞分化抗原分化群第133号）、CD44（白细胞分化抗原分化群第44号）的表达情况，应用SPSS 14.0统计软件进行数据分析，采用χ2检验统计方法。结果 sFRP、WIF-1、CD44、CD133基因在食管癌组织阳性表达率为32.50%，42.50%，60.00%，72.50%，在癌旁组织阳性表达率为65.00%，70.00%，37.50%，47.50%。 CD44、CD133基因在食管癌组织中表达水平高于癌旁组织（P<0.05），sFRP、WIF-1基因则在食管癌组织中表达水平低于癌旁组织（P<0.05），差异有统计学意义。结论sFRP、WIF-1在食管癌组织中表达低于癌旁组织，CD44、CD133在食管癌组织中表达高于癌旁组织。 sFRP、WIF-1基因低表达及CD44、CD133基因高表达可能与食管癌的发生、发展有关。
目的：探討sFRP、WIF-1、CD133、CD44在食管癌中的錶達與臨床病理特徵之間的關繫，為食管癌的研究提供新思路。方法選取2013年1月-2013年8月經手術切除的食管癌標本40例，術後病理均證實為食管癌患者，採用RT-PCR方法分析sFRP（分泌型捲麯相關蛋白）、WIF-1（Wnt抑製因子-1）、CD133（白細胞分化抗原分化群第133號）、CD44（白細胞分化抗原分化群第44號）的錶達情況，應用SPSS 14.0統計軟件進行數據分析，採用χ2檢驗統計方法。結果 sFRP、WIF-1、CD44、CD133基因在食管癌組織暘性錶達率為32.50%，42.50%，60.00%，72.50%，在癌徬組織暘性錶達率為65.00%，70.00%，37.50%，47.50%。 CD44、CD133基因在食管癌組織中錶達水平高于癌徬組織（P<0.05），sFRP、WIF-1基因則在食管癌組織中錶達水平低于癌徬組織（P<0.05），差異有統計學意義。結論sFRP、WIF-1在食管癌組織中錶達低于癌徬組織，CD44、CD133在食管癌組織中錶達高于癌徬組織。 sFRP、WIF-1基因低錶達及CD44、CD133基因高錶達可能與食管癌的髮生、髮展有關。
목적：탐토sFRP、WIF-1、CD133、CD44재식관암중적표체여림상병리특정지간적관계，위식관암적연구제공신사로。방법선취2013년1월-2013년8월경수술절제적식관암표본40례，술후병리균증실위식관암환자，채용RT-PCR방법분석sFRP（분비형권곡상관단백）、WIF-1（Wnt억제인자-1）、CD133（백세포분화항원분화군제133호）、CD44（백세포분화항원분화군제44호）적표체정황，응용SPSS 14.0통계연건진행수거분석，채용χ2검험통계방법。결과 sFRP、WIF-1、CD44、CD133기인재식관암조직양성표체솔위32.50%，42.50%，60.00%，72.50%，재암방조직양성표체솔위65.00%，70.00%，37.50%，47.50%。 CD44、CD133기인재식관암조직중표체수평고우암방조직（P<0.05），sFRP、WIF-1기인칙재식관암조직중표체수평저우암방조직（P<0.05），차이유통계학의의。결론sFRP、WIF-1재식관암조직중표체저우암방조직，CD44、CD133재식관암조직중표체고우암방조직。 sFRP、WIF-1기인저표체급CD44、CD133기인고표체가능여식관암적발생、발전유관。
Objective To discuss the relationship between the expressions of sFRP,WIF-1,CD133 and CD44 in esophageal cancer and its clinicopathological features,in order to provide new ideas for the study of esophageal cancer. Methods 40 cases of patients with esophageal cancer who suffered excision from January to August 2013 were selected. All the patients were confirmed with esophageal cancer by postoperative pathology. Expressions of sFRP (Secreted Frizzled Related Protein),WIF-1 (Wnt inhibitory factor-1),CD133 (cluster of differentiation 133) and CD44 (cluster of differentiation 44) were analyzed by RT-PCR,and data anal-ysis were made by SPSS 14.0 statistical software with statistical method χ2 test. Results Positive expression rates of sFRP genes, WIF-1 gene,CD44 gene and CD133 gene in esophageal cancer tissues were 32.50%,42.50%,60.00%,72.50%,respectively,while in adjacent tissues were65.00%,70.00%,37.50%,47.50%,respectively. Expressions of CD44 and CD133 gene were higher in esophageal cancer tissues than those in adjacent tissues(P<0.05),in contrast,expressions of sFRP genes and WIF-1 gene were low-er in esophageal cancer tissues than those in adjacent tissues (P<0.05),and the differences were statistically significant.Conclu-sion Expressions of sFRP and WIF-1 in esophageal cancer tissue were lower than those in adjacent tissues,while expressions of CD44 and CD133 in esophageal cancer tissue were higher than those in adjacent tissues. Low expressions of sFRP and WIF-1 genes and high expressions of CD44 and CD133 genes may be associated with the occurrence and development of esophageal can-cer.
