中国医药导报
中國醫藥導報
중국의약도보
CHINA MEDICAL HERALD
2014年
36期
25-29
,共5页
乳腺癌%表皮生长因子受体%B淋巴细胞瘤-2基因相关抗凋亡基因
乳腺癌%錶皮生長因子受體%B淋巴細胞瘤-2基因相關抗凋亡基因
유선암%표피생장인자수체%B림파세포류-2기인상관항조망기인
Breast cancer%EGFR%BAG-1
目的：探讨乳腺癌BAG-1基因表达与表皮生长因子受体(EGFR)表达的相关性。方法培养乳腺癌细胞株，采用实时聚合酶链反应技术检测EGFR(+)和EGFR(-)细胞株中BAG-1 mRNA的表达情况，West blot法检测其蛋白水平。分析BAG-1基因表达与EGFR表达的相关性。结果 MDA-MB-231、T47D细胞株EGFR mRNA和EGFR蛋白阳性表达，MCF-7、SLBP3细胞株EGFR mRNA和EGFR蛋白阴性表达。T47D细胞株BAG-1 mRNA的表达水平最高[(29.6±0.4)×10-4]，其次为MDA-MB-231细胞株[(6.9±0.3)×10-4]，两者的表达水平均显著高于MCF-7细胞株[(4.3±0.5)×10-4]、SKBR3细胞株[(0.3±0.1)×10-4]，差异的有高度统计学意义(P<0.01)。结论 BAG-1表达与EGFR存在相关性，可作为乳腺癌靶向治疗的潜在靶点。
目的：探討乳腺癌BAG-1基因錶達與錶皮生長因子受體(EGFR)錶達的相關性。方法培養乳腺癌細胞株，採用實時聚閤酶鏈反應技術檢測EGFR(+)和EGFR(-)細胞株中BAG-1 mRNA的錶達情況，West blot法檢測其蛋白水平。分析BAG-1基因錶達與EGFR錶達的相關性。結果 MDA-MB-231、T47D細胞株EGFR mRNA和EGFR蛋白暘性錶達，MCF-7、SLBP3細胞株EGFR mRNA和EGFR蛋白陰性錶達。T47D細胞株BAG-1 mRNA的錶達水平最高[(29.6±0.4)×10-4]，其次為MDA-MB-231細胞株[(6.9±0.3)×10-4]，兩者的錶達水平均顯著高于MCF-7細胞株[(4.3±0.5)×10-4]、SKBR3細胞株[(0.3±0.1)×10-4]，差異的有高度統計學意義(P<0.01)。結論 BAG-1錶達與EGFR存在相關性，可作為乳腺癌靶嚮治療的潛在靶點。
목적：탐토유선암BAG-1기인표체여표피생장인자수체(EGFR)표체적상관성。방법배양유선암세포주，채용실시취합매련반응기술검측EGFR(+)화EGFR(-)세포주중BAG-1 mRNA적표체정황，West blot법검측기단백수평。분석BAG-1기인표체여EGFR표체적상관성。결과 MDA-MB-231、T47D세포주EGFR mRNA화EGFR단백양성표체，MCF-7、SLBP3세포주EGFR mRNA화EGFR단백음성표체。T47D세포주BAG-1 mRNA적표체수평최고[(29.6±0.4)×10-4]，기차위MDA-MB-231세포주[(6.9±0.3)×10-4]，량자적표체수평균현저고우MCF-7세포주[(4.3±0.5)×10-4]、SKBR3세포주[(0.3±0.1)×10-4]，차이적유고도통계학의의(P<0.01)。결론 BAG-1표체여EGFR존재상관성，가작위유선암파향치료적잠재파점。
Objective To discuss the correlation of BAG-1 gene expression and EGFR expression in breast cancer. Methods Breast cancer cell lines were cultured, BAG-1 mRNA expression was detected by PCR in EGFR (+) and EGFR (-) cell lines. BAG-1 protein expression was detected by West blot. Correlation of BAG-1 gene expression and EGFR expression in breast cancer was analyzed. Results EGFR mRNA and EGFR protein were positive expression in EGFR MDA-MB-231 and T47D cell lines, and negative expression in MCF-7 and SLBP3 cell lines. BAG-1 mRNA expres-sion was highest in T47D cell line [(29.6±0.4)í10-4], followed by MDA-MB-231 cell line [(6.9±0.3)í10-4]. Both expres-sion levels were significantly higher than that of MCF-7 cell line [(4.3±0.5)í10-4] and SKBR3 cell line [(0.3±0.1)í10-4], the differences were statistically significant (P<0.01). Conclusion Expression of BAG-1 shows correlation with EGFR, which can be used as a potential targets for targeted therapy of breast cancer.
