CAJ | 학술논문

目的：研究膜型基质金属蛋白酶-1(MT1-MMP)表达对肝细胞癌血管生成拟态(VM)形成的影响及机制。方法构建靶向MT1-MMP的RNA干扰质粒载体，转染肝癌细胞系Bel7402，实时定量PCR及Western blot检测干扰效果；在体外三维细胞培养体系中观察其对VM管状结构形成的影响；Transell侵袭小室实验检测细胞侵袭能力。结果靶向抑制MT1-MMP的表达下调肿瘤细胞侵袭能力，影响肝癌细胞在三维细胞培养体系中VM管状结构的形成。结论 MT1-MMP表达影响肝细胞癌VM的形成，可能成为肝细胞癌抗血管生成治疗的新靶点。
목적：연구막형기질금속단백매-1(MT1-MMP)표체대간세포암혈관생성의태(VM)형성적영향급궤제。방법구건파향MT1-MMP적RNA간우질립재체，전염간암세포계Bel7402，실시정량PCR급Western blot검측간우효과；재체외삼유세포배양체계중관찰기대VM관상결구형성적영향；Transell침습소실실험검측세포침습능력。결과파향억제MT1-MMP적표체하조종류세포침습능력，영향간암세포재삼유세포배양체계중VM관상결구적형성。결론 MT1-MMP표체영향간세포암VM적형성，가능성위간세포암항혈관생성치료적신파점。
Objective To investigate the effect of MT1-MMP on vasculogenic mimicry (VM) formation in hepatocellular carcinoma (HCC) and discuss its possible mechanisms. Methods RNA interfere plasmid targeting MT1-MMP was con-structed and transfected into a HCC cell lines. Interference effect was detected by real time PCR and Western blot. VM networks in 3D cell culture system were observed with a microscope. Cell invasion assay was performed using 24-well transwell. Results Inhibition of MT1-MMP expression impaired VM network formation in vitro by down-regulating cell invasion. Conclusion MT1-MMP influences vasculogenic mimicry formation in HCC cell line and offers a potential molecular target for HCC antiangiogenesis therapy.