CAJ | 학술논문

为了解和分析南海沿岸地区临床分离的哈维弧菌(Vibrio harveyi)菌株耐药谱多样性,采用K-B法对84株哈维弧菌分离株进行了12种抗生素的敏感性实验,并通过聚类分析对分离菌株耐药性进行研究,探讨其抗生素耐药性同菌株时空分布的关系。实验结果显示,84株哈维弧菌的耐药谱型数量为26种,谱型丰富度为31.0%；多重耐药谱型数量(耐药种数3种以上)为13种,占总耐药谱型数量的50.0%,谱型丰富度达59.1%。海南、广东、广西、福建的分离株分别包含有18种、15种、3种、1种耐药谱型,4个地区均具有J型(FUR/AMO)耐药谱；2007―2012年各年份菌株耐药谱型为：2007年2种(J、M,耐2种抗生素)、2010年4种(A、B、J、M,耐5种抗生素)、2011年24种(除Y、Z外的A-X耐药谱型,耐7种抗生素),2012年26种(包括Y、Z耐药谱型,耐8种抗生素)。聚类分析将实验菌株分为i~vi共6个亚群,进而聚类为Group I、Group II两个组群；各亚群菌株具有独特的耐药谱型,耐药谱型分别是N、P、T、U、Y(i亚群), R、S(ii亚群), K、P(iii亚群), F、H、O、X、V、W(iv亚群), E、G、Q(v亚群), C、L、Z(vi 亚群)；两个组群菌株没有共同的耐药谱型。研究结果表明,哈维弧菌分离株耐药谱型多样,谱型丰富度较高,来源不同的菌株耐药谱型存在差异,聚类分析方法能够根据耐药性情况有效地对菌株分型。
위료해화분석남해연안지구림상분리적합유호균(Vibrio harveyi)균주내약보다양성,채용K-B법대84주합유호균분리주진행료12충항생소적민감성실험,병통과취류분석대분리균주내약성진행연구,탐토기항생소내약성동균주시공분포적관계。실험결과현시,84주합유호균적내약보형수량위26충,보형봉부도위31.0%；다중내약보형수량(내약충수3충이상)위13충,점총내약보형수량적50.0%,보형봉부도체59.1%。해남、엄동、엄서、복건적분리주분별포함유18충、15충、3충、1충내약보형,4개지구균구유J형(FUR/AMO)내약보；2007―2012년각년빈균주내약보형위：2007년2충(J、M,내2충항생소)、2010년4충(A、B、J、M,내5충항생소)、2011년24충(제Y、Z외적A-X내약보형,내7충항생소),2012년26충(포괄Y、Z내약보형,내8충항생소)。취류분석장실험균주분위i~vi공6개아군,진이취류위Group I、Group II량개조군；각아군균주구유독특적내약보형,내약보형분별시N、P、T、U、Y(i아군), R、S(ii아군), K、P(iii아군), F、H、O、X、V、W(iv아군), E、G、Q(v아군), C、L、Z(vi 아군)；량개조군균주몰유공동적내약보형。연구결과표명,합유호균분리주내약보형다양,보형봉부도교고,래원불동적균주내약보형존재차이,취류분석방법능구근거내약성정황유효지대균주분형。
The present study investigated the antibiogram types of Vibrio harveyi isolates collected from diseased fish that were cultured along the south China coast. The antibiotic resistance profiles of 84 strains were tested with 12 common antibiotics using Kirby–Bauer disc diffusion methodology. The antimicrobial susceptibility data were used to identify correlations between antibiogram and subgroup types using cluster analysis software (SPSS 19.0, Statistical Product and Service Solutions). Eighty-four V. harveyi isolates formed 26 antibiogram types with an antibiogram abundance of 31.0%. The number of multiple antibiotic resistant types (i.e., resistant to more than three antibiotics) in the strains was 13 and comprised 50.0% of the total antibiogram types, causing the multi-antibiogram abundance value to increase to 59.1%. Isolates originating from Hainan, Guangdong, Guangxi, and Fujian Provinces possessed 18, 15, 3, and 1 of the antibiogram types, respectively, and all shared the J antibiogram type (FUR/AMO). Representative antibiogram types were J and M from 2007, A and B were added in 2010, 24 A to X types (A to X except for Y, Z) were added in 2011, while Y and Z appeared in 2012. The kinds of suppressive antimicrobial agents was 2, 5, 7, and 8, thereby mirroring the sampling years, 2007, 2010, 2011 and 2012, respectively. Strains isolated from Epinephelus awoara, E. fuscoguttatus, Plectropomus leopardus, Trachinotus ovatus and E. lanceolatus♂ × E. fuscoguttatus♀ contained 6–10 antibiogram types, while the remaining fish sampled had only 1–3 types. The strains clustered initially into six subgroups (i–vi) and further formed groups I and II. Each subgroup contained characteristic antibiogram types comprising N, P, T, U, Y;R, S;K, P; F, H, O, X, V, W; E, G, Q; and C, L, Z. Common antibiogram types were not found in the Group I and II strains. Our research revealed that the V. harveyi isolates displayed polymorphic antibiogram types of moderately high abundance and could be typed for resistance effectively by cluster analysis. The resistance patterns of the isolates gathered from different sources were diverse.