CAJ | 학술논문

目的：探讨真核细胞起始因子-4E(eIF-4E)在非小细胞肺癌(NSCLC)组织中的表达情况及其与预后的关系。方法选择2009年1月~2010年12月温州医科大学附属黄岩医院外科手术切除的NSCLC组织60例以及30例癌旁正常组织样本作为对照，采用免疫组化法检测NSCLC组织以及癌旁正常肺组织中elF4E的表达。NSCLC患者术后均接受2周以上的榄香烯联合顺铂化疗，对所有患者均进行系统地随访评价，分析eIF-4E的表达与无瘤生存期(DFS)和总生存时间(OS)的关系。结果 eIF-4E在NSCLC癌组织中的表达率为80.00%，显著高于正常癌旁组织(33.33%)，差异有高度统计学意义(P<0.01)。年龄≥60岁及TNM分期为Ⅲ~Ⅳ期患者的eIF-4E高表达率明显高于年龄<60岁及分期为I~Ⅱ期的患者，差异均有统计学意义(P<0.05)。 eIF-4E低表达患者的DFS和OS均显著高于高表达者，差异有统计学意义(P<0.05)。结论 eIF-4E基因在NSCLC组织中呈高水平表达，榄香烯联合顺铂化疗后eIF-4E低表达患者的DFS和OS明显延长。 eIF-4E高表达水平可作为NSCLC不良预后的预测因子。
목적：탐토진핵세포기시인자-4E(eIF-4E)재비소세포폐암(NSCLC)조직중적표체정황급기여예후적관계。방법선택2009년1월~2010년12월온주의과대학부속황암의원외과수술절제적NSCLC조직60례이급30례암방정상조직양본작위대조，채용면역조화법검측NSCLC조직이급암방정상폐조직중elF4E적표체。NSCLC환자술후균접수2주이상적람향희연합순박화료，대소유환자균진행계통지수방평개，분석eIF-4E적표체여무류생존기(DFS)화총생존시간(OS)적관계。결과 eIF-4E재NSCLC암조직중적표체솔위80.00%，현저고우정상암방조직(33.33%)，차이유고도통계학의의(P<0.01)。년령≥60세급TNM분기위Ⅲ~Ⅳ기환자적eIF-4E고표체솔명현고우년령<60세급분기위I~Ⅱ기적환자，차이균유통계학의의(P<0.05)。 eIF-4E저표체환자적DFS화OS균현저고우고표체자，차이유통계학의의(P<0.05)。결론 eIF-4E기인재NSCLC조직중정고수평표체，람향희연합순박화료후eIF-4E저표체환자적DFS화OS명현연장。 eIF-4E고표체수평가작위NSCLC불량예후적예측인자。
Objective To investigate eIF-4E expression in patients with non-small cell lung cancer (NSCLC), and its correlation with prognosis. Methods Tumor tissues were collected from 60 NSCLC patients who underwent surgical re-section from January 2009 to December 2010 in the Affiliated Huangyan Hospital of Wenzhou Medical University, and 30 adjacent normal tissues samples were also collected. The expression level of eIF-4E in NSCLC tissues and adjacent normal tissues were measured by immunohistochemical method. All the NSCLC patients after resection were subject to treatment with elemene plus cisplatin for more than 2 weeks. A systematic follow-up evaluation was also performed to investigate the correlation of eIF-4E expression level with disease-free survival (DFS) and overall survival (OS). Re-sults The expression rate of eIF-4E in the tumor tissues (80.00%) was significantly higher than that in adjacent normal tissues (33.33%), the difference was statistically significant (P< 0.01). The eIF-4E high expression rate of the patient with age≥60 years and clinical stage of Ⅲ-Ⅳ was higher than whose age<60 years and clinical stage ofⅠ-II, the differences were statistically significant (P < 0.05). The DFS and OS values of patients with low eIF-4E expression level were significantly larger than those of patients with high eIF-4E expression level, the differences were statistically significant (P< 0.05). Conclusion eIF-4E is highly expressed in NSCLC tissues; the DFS and OS values of patients with low eIF-4E expression level are significantly larger. High eIF-4E expression level may be an independent predic-tor of poor NSCLC prognosis.