CAJ | 학술논문

目的：研究白色念珠菌对人口腔黏膜上皮角质细胞的作用机制。方法将培养的口腔黏膜上皮角质细胞分别加入孢子型白色念珠菌(孢子组)和菌丝型白色念珠菌(菌丝组)，另将单独培养的口腔黏膜上皮角质细胞作为阴性对照组。比较各组对口腔黏膜上皮角质细胞的黏附作用，并观察白色念珠菌对口腔黏膜上皮角质细胞的凋亡和增殖作用。结果菌丝组黏附指数(7.63±1.39)显著高于孢子组(3.47±1.04)，差异有统计学意义(P<0.05)；菌丝组凋亡率[(3.49±0.4)%]显著高于孢子组[(2.07±0.15)%]和阴性对照组[(1.98±0.07)%]，差异有统计学意义(P<0.05)，而孢子组和阴性对照组凋亡率相比差异无统计学意义(P>0.05)。菌丝组G0/G1期细胞比例[(38.17±7.83)%]显著低于孢子组[(49.47±11.41)%]和阴性对照组[(57.71±9.39)%]，差异有统计学意义(P <0.05)；S期[(8.54±4.03)%]、G2/M期[(20.18±9.59)%]细胞比例上升，且显著高于孢子组[(6.47±2.73)%、(10.71±3.19)%]和阴性对照组[(5.35±2.11)%、(12.38±4.35)%]，差异有统计学意义(P<0.05)；PI[(42.79±18.73)%]明显高于孢子组[(28.67±8.79)%]和阴性对照组[(26.87±7.64)%]，差异有统计学意义(P<0.05)，而孢子组和阴性对照组PI相比差异无统计学意义(P>0.05)。结论白色念珠菌能够导致口腔黏膜上皮角质细胞发生凋亡和增殖周期改变。
목적：연구백색념주균대인구강점막상피각질세포적작용궤제。방법장배양적구강점막상피각질세포분별가입포자형백색념주균(포자조)화균사형백색념주균(균사조)，령장단독배양적구강점막상피각질세포작위음성대조조。비교각조대구강점막상피각질세포적점부작용，병관찰백색념주균대구강점막상피각질세포적조망화증식작용。결과균사조점부지수(7.63±1.39)현저고우포자조(3.47±1.04)，차이유통계학의의(P<0.05)；균사조조망솔[(3.49±0.4)%]현저고우포자조[(2.07±0.15)%]화음성대조조[(1.98±0.07)%]，차이유통계학의의(P<0.05)，이포자조화음성대조조조망솔상비차이무통계학의의(P>0.05)。균사조G0/G1기세포비례[(38.17±7.83)%]현저저우포자조[(49.47±11.41)%]화음성대조조[(57.71±9.39)%]，차이유통계학의의(P <0.05)；S기[(8.54±4.03)%]、G2/M기[(20.18±9.59)%]세포비례상승，차현저고우포자조[(6.47±2.73)%、(10.71±3.19)%]화음성대조조[(5.35±2.11)%、(12.38±4.35)%]，차이유통계학의의(P<0.05)；PI[(42.79±18.73)%]명현고우포자조[(28.67±8.79)%]화음성대조조[(26.87±7.64)%]，차이유통계학의의(P<0.05)，이포자조화음성대조조PI상비차이무통계학의의(P>0.05)。결론백색념주균능구도치구강점막상피각질세포발생조망화증식주기개변。
Objective To explore the mechanism of Candida albicans on human buccal epithelial cells. Methods The oral mucosa keratinocyte cultures were added to spore type of Candida albicans (spores group) and hyphae of Candida albicans (mycelium group), oral mucosal epithelial cells cultured alone as negative control group. Compared adhesion effect of oral mucosal epithelial cells in each group, and observed the apoptosis and proliferation of Candida albicans to buccal epithelial cells. Results Adhesion index of mycelium group (7.63±1.39) was significantly higher than spore group (3.47±1.04), with statistically significant difference (P< 0.05); the apoptosis rate [(3.49±0.4)%] was significantly higher than that in spores group [(2.07±0.15)%] and the control group [(1.98±0.07)%], with statistically significant dif-ference (P<0.05), while the spores group and the control group had no significant difference (P>0.05). The proportion of phase G0/G1 in mycelium group [(38.17±7.83)%] was significantly lower than that of spore group [(49.47±11.41)%] and the control group [(57.71±9.39)%], with statistically significant difference (P<0.05);the proportion of phase S [(8.54±4.03)%] and phase G2/M [(20.18±9.59)%] increased, significantly higher than those of the spores group [(6.47±2.73)%, (10.71±3.19)%] and the control group [(5.35±2.11)%, (12.38±4.35)%], with statistically significant difference (P< 0.05);PI [(42.79±18.73)%] was significantly higher than spore group [(28.67±8.79)%] and the control group [(26.87±7.64)%] , with statistically significant difference (P<0.05), while the spores group and the control group had no significant difference (P>0.05). Conclusion Candida albicans can cause oral mucosa keratinocyte apoptosis and proliferation cycle change.